Global Identification of Anti-Melanoma Cellular Targets by Photochemically Induced Coupling of Reactions on the Surface of Magnetic Particles.

, an active component of Arnebia euchroma (Royle) Johnst., has remarkable pharmacological effects, particularly in its anti-tumour activity. Nonetheless, the specific targets and mechanisms of action remain to be further explored. A novel FeO@ was designed and synthesized in this study by linking FeO and through benzophenone. FeO@ was characterized using several techniques, including scanning electron microscopy (SEM), Fourier-transform infrared spectroscopy (FT-IR), and drug removal methods, to determine the content of on the surface of the magnetic particles. Target hooking technology was utilized to identify the target proteins of the compound in melanoma. The synthesized FeO@ was co-incubated with A375 cell lysate, followed by the target proteins, which were purified by magnetic enrichment using magnetic microspheres and identified by high-resolution mass spectrometry. AutoDock Vina software was employed for molecular docking analysis, where it was found that targets RPN1, CPEB4, and HNRNPUL1 proteins. Subsequently, A375 cells were treated with at different concentrations (2.5, 5.0, 10.0 μM) for 48 h, and the expressions of the three proteins were observed. The results showed a significant reduction in the relative expression of CPEB4 in the high-dose group compared to the control group ( < 0.01). Moreover, the relative expression of HNRNPUL1 was decreased in the medium- and high-dose groups ( < 0.05). This study initially revealed from the source that may regulate melanoma-specific markers, melanosomes, tyrosine kinases related to abnormal tyrosine metabolism, and melanoma through multiple targets such as CPEB4 and HNRNPUL1. Proliferation and metastasis work together to exert an anti-melanoma mechanism, which provides a new idea for the follow-up study of the molecular pharmacological mechanism of the complex system of total naphthoquinones in (Royle) Johns.