Zhang Pu, Wang Fang, Zhu Chashan
College of Agronomy, Xinjiang Agricultural University, Urumqi 830052, Xinjiang, China.
Sheng Wu Gong Cheng Xue Bao. 2013 Feb;29(2):214-23.
In order to investigate the effects of fungal elicitor and macroporous adsorption resin on shikonin accumulation in hairy roots of arnebia euchroma (Royle) Johnst, we used spectrophotometry to determine the total naphthoquinone content of the hairy roots, by adding different volume ratio of Aspergillus niger elicitor, Aspergillus oryzae elicitor, and the macroporous resin into the M-9 liquid medium at different culture time. The results show that the total naphthoquinone content was 2.28 times higher than the control when we added mixed elicitors of Aspergillus niger and Aspergillus oryzae at the ratio of 2.5:50 in the 10th day of hairy roots cultivating. The total naphthoquinone content was 3.71 times higher than that of the control, when we added macroporous adsorption resin NKA-9. Aspergillus niger elicitor exhibited synergistic effect with Aspergillus oryzae elicitor to enhance the naphthoquinone. Also, the total naphthoquinone level was 4.17 times higher than that of the control by adding mixed fungal elicitor and macroporous adsorption resin NKA-9 in the bioreactor. Aspergillus oryzae and mixed elicitor could promote the hairy roots proliferation, and macroporous adsorption resin NKA-9 and mixed elicitor increased the total naphthoquinone content. In summary, the measure developed for Arnebia euchroma (Royle) Johnst hairy roots cultivating in bioreactors may potential for large-scale production of naphthoquinone.
为了研究真菌诱导子和大孔吸附树脂对新疆紫草毛状根中紫草素积累的影响，我们采用分光光度法测定毛状根中总萘醌含量，即在不同培养时间向M-9液体培养基中添加不同体积比的黑曲霉诱导子、米曲霉诱导子和大孔树脂。结果表明，在毛状根培养第10天，当以2.5:50的比例添加黑曲霉和米曲霉混合诱导子时，总萘醌含量比对照高2.28倍。当添加大孔吸附树脂NKA-9时，总萘醌含量比对照高3.71倍。黑曲霉诱导子与米曲霉诱导子表现出协同作用以提高萘醌含量。此外，在生物反应器中添加混合真菌诱导子和大孔吸附树脂NKA-9时，总萘醌水平比对照高4.17倍。米曲霉和混合诱导子可促进毛状根增殖，大孔吸附树脂NKA-9和混合诱导子可提高总萘醌含量。总之，所开发的用于新疆紫草毛状根在生物反应器中培养的方法可能具有大规模生产萘醌的潜力。
