Hernández Jesús, Henao-Díaz Alexanda, Reséndiz-Sandoval Mónica, Cota-Valdez Angel, Mata-Haro Verónica, Gimenez-Lirola Luis G
Laboratorio de Inmunología, Centro de Investigación en Alimentación y Desarrollo, A.C., Hermosillo 83304, SON, Mexico.
Grupo Bachoco, Unidad de Negocios Cerdo, Celaya 38000, GTO, Mexico.
Vaccines (Basel). 2024 Nov 26;12(12):1318. doi: 10.3390/vaccines12121318.
This study investigated the dynamics of porcine circovirus type 2 (PCV2) and PCV3 on a commercial farm following PCV2 vaccination.
Serum samples from 35 pigs, starting at 3 weeks of age, were collected weekly until 21 weeks of age. Oral fluids from six pens of pigs of the same age were also analyzed. Viral DNA was assessed in pooled sera and individual oral fluid samples, while antibodies (IgG and IgA) were measured in the serum and oral fluids. Productive parameters, including weekly mortality and cumulative mortality, were evaluated.
The results revealed that PCV2 and PCV3 co-infection was detected in pigs at 8 weeks of age, with PCV3 being detected in oral fluids two weeks earlier. PCV3 DNA was detected in oral fluids at 4 weeks of age. PCV2 IgG antibodies in the serum increased gradually after vaccination, peaking at 7 weeks of age, then declined and stabilized until 21 weeks of age. PCV3 IgG antibodies fluctuated early but were uniformly positive after 13 weeks of age. In oral fluids, PCV2 IgG and IgA antibodies showed a strong response only at 3 and 23 weeks of age. In contrast, a strong and consistent IgG response was observed in oral fluids in the absence of PCV2 and PCV3 co-infection of pigs at 3 to 11 weeks of age. The farm's productive parameters remained stable throughout the study.
These findings suggest that PCV2 and PCV3 co-infection, along with high PCV3 detection levels in serum and oral fluids, may have an impact on the efficacy of PCV2 vaccination.
本研究调查了猪圆环病毒2型（PCV2）疫苗接种后，一家商业猪场中PCV2和PCV3的动态变化。
从35头3周龄的猪开始，每周采集血清样本，直至21周龄。还对来自六个同龄猪栏的口腔液体进行了分析。对混合血清和个体口腔液体样本中的病毒DNA进行评估，同时在血清和口腔液体中检测抗体（IgG和IgA）。评估了包括每周死亡率和累积死亡率在内的生产参数。
结果显示，8周龄的猪中检测到PCV2和PCV3共同感染，PCV3在口腔液体中提前两周被检测到。4周龄时在口腔液体中检测到PCV3 DNA。接种疫苗后血清中的PCV2 IgG抗体逐渐增加，在7周龄时达到峰值，然后下降并稳定至21周龄。PCV3 IgG抗体早期波动，但在13周龄后均为阳性。在口腔液体中，PCV2 IgG和IgA抗体仅在3周龄和23周龄时出现强烈反应。相比之下，在3至11周龄未发生PCV2和PCV3共同感染的猪的口腔液体中观察到强烈且一致的IgG反应。在整个研究过程中，该猪场的生产参数保持稳定。
这些发现表明，PCV2和PCV3共同感染，以及血清和口腔液体中PCV3的高检测水平，可能会影响PCV2疫苗接种的效果。
