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Development Using Bioluminescence Imaging of a Recombinant Anguillid Herpesvirus 1 Vaccine Candidate Associated with Normal Replication In Vitro but Abortive Infection In Vivo.

作者信息

Zhang Haiyan, Sridhar Arun, Delrez Natacha, He Bo, Fourny Sophie, Gao Yuan, Donohoe Owen, Vanderplasschen Alain F C

机构信息

Immunology-Vaccinology, Department of Infectious and Parasitic Diseases, Fundamental and Applied Research for Animals & Health (FARAH), Faculty of Veterinary Medicine, University of Liège, B-4000 Liège, Belgium.

Bioscience Research Institute, Technological University of the Shannon, N37 HD68 Athlone, Westmeath, Ireland.

出版信息

Vaccines (Basel). 2024 Dec 17;12(12):1423. doi: 10.3390/vaccines12121423.

DOI:10.3390/vaccines12121423
PMID:39772083
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11728778/
Abstract

BACKGROUND/OBJECTIVES: Anguillid herpesvirus 1 (AngHV-1) (recently renamed Cyvirus anguillidallo 1) is the etiologic agent of a lethal disease that affects several eel species. It is thought to be one of the main infectious agents causing a population decline in wild eels and economic loss within the eel aquaculture sector. To date, no vaccines are available against AngHV-1. Recently, we developed a safe and efficacious live attenuated recombinant vaccine against Cyprinid herpesvirus 3 (CyHV-3). This CyHV-3 recombinant vaccine encodes a deletion of ORF57. Orthologues of CyHV-3 ORF57 exist in Cyprinid herpesvirus 2 (CyHV-2, ORF57) and AngHV-1 (ORF35). METHODS: In the present study, using recombinant strains and bioluminescent in vivo imaging, we investigated the effect of AngHV-1 ORF35 deletion on virus replication in vitro, virulence in vivo, and the potential of an AngHV-1 ORF35-deleted recombinant as a vaccine candidate for the mass vaccination of eels by immersion. With this goal in mind, we produced ORF35-deleted recombinants using two parental strains: a UK strain and a recombinant derived from the former strain by insertion of a Luciferase-GFP reporter cassette into a non-coding intergenic region. RESULTS: Analyses of ORF35-deleted recombinants led to the following observations: (i) AngHV-1 ORF35 is not essential for viral growth in cell culture, and its deletion does not affect the production of extracellular virions despite reducing the size of viral plaque. (ii) In contrast to what has been observed for CyHV-3 ORF57 and CyHV-2 ORF57, in vivo bioluminescent analyses revealed that AngHV-1 ORF35 is an essential virulence factor and that its deletion led to abortive infection in vivo. (iii) Inoculation of the AngHV-1 ORF35-deleted recombinant by immersion induced a protective immune response against a wild-type challenge. This protection was shown to be dose-dependent and to rely on the infectivity of AngHV-1 ORF35-deleted virions. CONCLUSIONS: This study suggests that the AngHV-1 ORF35 protein has singular properties compared to its orthologues encoded by CyHV-2 and CyHV-3. It also supports the potential of AngHV-1 ORF35-deleted recombinants for the mass vaccination of eels by immersion.

摘要
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bf13/11728778/138a44e4325a/vaccines-12-01423-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bf13/11728778/b02a9fafff77/vaccines-12-01423-g003a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bf13/11728778/5359ea05d1b1/vaccines-12-01423-g004a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bf13/11728778/21410ee1bb5d/vaccines-12-01423-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bf13/11728778/138a44e4325a/vaccines-12-01423-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bf13/11728778/b02a9fafff77/vaccines-12-01423-g003a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bf13/11728778/5359ea05d1b1/vaccines-12-01423-g004a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bf13/11728778/21410ee1bb5d/vaccines-12-01423-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bf13/11728778/138a44e4325a/vaccines-12-01423-g002.jpg

相似文献

[1]
Development Using Bioluminescence Imaging of a Recombinant Anguillid Herpesvirus 1 Vaccine Candidate Associated with Normal Replication In Vitro but Abortive Infection In Vivo.

Vaccines (Basel). 2024-12-17

[2]
Identification of an essential virulence gene of cyprinid herpesvirus 3.

Antiviral Res. 2017-9

[3]
Rational development of an attenuated recombinant cyprinid herpesvirus 3 vaccine using prokaryotic mutagenesis and in vivo bioluminescent imaging.

PLoS Pathog. 2015-2-20

[4]
Proteomic and Functional Analyses of the Virion Transmembrane Proteome of Cyprinid Herpesvirus 3.

J Virol. 2017-10-13

[5]
Development of a loop-mediated isothermal amplification (LAMP) assay for rapid and visual detection of Anguillid herpesvirus 1.

J Virol Methods. 2024-12

[6]
Immune responses and protective efficacy of American eel (Anguilla rostrata) immunized with a formalin-inactivated vaccine against Anguillid herpesvirus.

Fish Shellfish Immunol. 2024-1

[7]
Generation and Characterization of ORF55/ORF57-Deleted Recombinant 2 Mutants with Chimeric Capsid Protein Gene of Grouper Nervous Necrosis Virus.

Vaccines (Basel). 2023-12-30

[8]
Isolation and Identification of a New Isolate of Anguillid Herpesvirus 1 from Farmed American Eels () in China.

Viruses. 2022-12-7

[9]
Anguillid herpesvirus 1 (AngHV) ORF95 encodes a late, structural envelope protein.

Virus Genes. 2021-6

[10]
Glass eels and viruses - a lesson learnt from stocking the eastern German Baltic Sea coast.

Transbound Emerg Dis. 2022-11-16

引用本文的文献

[1]
Establishment and Partial Characterization of Three Novel Permanent Cell Lines Originating from European Freshwater Fish Species.

Pathogens. 2025-5-26

[2]
Genomic and Phenotypic Characterization of a Novel Virulent Strain of 2 Originating from an Outbreak in The Netherlands.

Viruses. 2025-4-30

本文引用的文献

[1]
Generation and Characterization of ORF55/ORF57-Deleted Recombinant 2 Mutants with Chimeric Capsid Protein Gene of Grouper Nervous Necrosis Virus.

Vaccines (Basel). 2023-12-30

[2]
Immune responses and protective efficacy of American eel (Anguilla rostrata) immunized with a formalin-inactivated vaccine against Anguillid herpesvirus.

Fish Shellfish Immunol. 2024-1

[3]
In Vivo Imaging Sheds Light on the Susceptibility and Permissivity of to Cyprinid Herpesvirus 2 According to Developmental Stage.

Viruses. 2023-8-15

[4]
Monitoring for Anguillicoloides crassus, Anguillid herpesvirus 1, aquabirnavirus EVE and rhabdovirus EVEX in the European eel population of southern Spain.

J Fish Dis. 2023-4

[5]
Isolation and Identification of a New Isolate of Anguillid Herpesvirus 1 from Farmed American Eels () in China.

Viruses. 2022-12-7

[6]
Anguilla sp. diseases diagnoses and treatments: The ideal methods at the crossroads of conservation and aquaculture purposes.

J Fish Dis. 2022-7

[7]
Active swimming and transphort by currents observed in Japanese eels (Anguilla japonica) acoustically tracked in the western North Pacific.

Sci Rep. 2022-3-1

[8]
Methods of Inactivation of Highly Pathogenic Viruses for Molecular, Serology or Vaccine Development Purposes.

Pathogens. 2022-2-19

[9]
A complete check-up of European eel after eight years of restocking in an upland river: Trends in growth, lipid content, sex ratio and health status.

Sci Total Environ. 2022-2-10

[10]
Genomes of Anguillid Herpesvirus 1 Strains Reveal Evolutionary Disparities and Low Genetic Diversity in the Genus .

Microorganisms. 2021-5-5

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