Doszpoly Andor
HUN-REN Veterinary Medical Research Institute, 1143 Budapest, Hungary.
Pathogens. 2025 May 26;14(6):531. doi: 10.3390/pathogens14060531.
The establishment and partial characterization of three continuous cell lines from European freshwater fish species are provided. The three new cell lines, designated NPL-3, AF-1, and PF-1, were created from larvae of northern pike ) and fin tissues of asp () and European perch () fin tissues, respectively. All three cell lines have been subcultured more than 90 times since their establishment. Cells were optimally maintained at 25 °C in M199 medium supplemented with 10% fetal bovine serum. The NPL-3 and AF-1 cells are susceptible to spring viraemia of carp virus, pike fry rhabdovirus, ictalurid herpesvirus 2, and European catfish virus, while in the PF-1 cells, only the latter two viruses were successfully propagated. These newly established cell lines could serve as diagnostic tools for the aforementioned economically important viral diseases. They might be effective appliances for isolating novel viruses from northern pike, asp, European perch, and other closely related fish species.
本文提供了来自欧洲淡水鱼物种的三种连续细胞系的建立及部分特性描述。这三种新细胞系分别命名为NPL - 3、AF - 1和PF - 1，它们分别由白斑狗鱼的幼体、赤梢鱼的鳍组织以及欧洲鲈鱼的鳍组织建立而成。自建立以来，这三种细胞系均已传代培养超过90次。细胞在补充有10%胎牛血清的M199培养基中于25°C条件下可得到最佳培养。NPL - 3和AF - 1细胞对鲤春病毒血症病毒、梭子鱼幼体弹状病毒、斑点叉尾鮰疱疹病毒2型以及欧洲鲶鱼病毒敏感，而在PF - 1细胞中，仅后两种病毒能够成功增殖。这些新建立的细胞系可作为上述具有经济重要性的病毒性疾病的诊断工具。它们可能是从白斑狗鱼、赤梢鱼、欧洲鲈鱼以及其他亲缘关系密切的鱼类物种中分离新型病毒的有效工具。
