联合佐剂增强重组H5血凝素疫苗对鸡高剂量病毒攻击的保护作用。

Combination Adjuvants Enhance Recombinant H5 Hemagglutinin Vaccine Protection Against High-Dose Viral Challenge in Chickens.

作者信息

He Yanjuan, Wang Jiaxin, Chi Lanyan, Dong Yajing, Chen Huixin, Meng Xiaocui, Liao Ming, Luo Yongwen, Fan Huiying

机构信息

College of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, China.

Key Laboratory of Veterinary Vaccine Innovation of the Ministry of Agriculture and Rural Affairs, Guangzhou 510642, China.

出版信息

Vaccines (Basel). 2024 Dec 23;12(12):1448. doi: 10.3390/vaccines12121448.

BACKGROUND

Recombinant avian influenza subunit vaccines often require adjuvants to enhance immune responses. This study aims to evaluate the immune-enhancing potential of seven combination adjuvants in specific pathogen-free (SPF) chickens.

METHODS

SPF chickens were vaccinated with combinations of ISA78VG and adjuvants, including Quil-A, CpG, and monophosphoryl lipid A (MPLA). Their immune responses were assessed using a vaccination and viral challenge protection model.

RESULTS

The combinations of ISA78VG with Quil-A, CpG&MPLA or CpG&Quil-A significantly enhanced antibody responses and provided cross-protection against the H5N8-20135 strain. The ISA78VG&MPLA and ISA78VG&CpG&MPLA combinations induced the stronger IFN-γ production, with CpG further amplifying the immune response. The ISA78VG&Quil-A formulation, in particular, stimulated rapid antibody responses, achieving a 100% seroconversion by day 14 and high titers of hemagglutination inhibition (HI) antibodies against both the recombinant HA antigen and the H5N6-20053 virus.

CONCLUSIONS

The ISA78VG&Quil-A combination is an ideal adjuvant for enhancing the immunogenicity of avian influenza rHA subunit vaccines, offering a promising strategy for H5 subtype vaccine development.

背景

重组禽流感亚单位疫苗通常需要佐剂来增强免疫反应。本研究旨在评估七种联合佐剂对无特定病原体（SPF）鸡的免疫增强潜力。

方法

用ISA78VG与包括Quil-A、CpG和单磷酰脂质A（MPLA）在内的佐剂组合对SPF鸡进行疫苗接种。使用疫苗接种和病毒攻击保护模型评估它们的免疫反应。

结果

ISA78VG与Quil-A、CpG&MPLA或CpG&Quil-A的组合显著增强了抗体反应，并提供了针对H5N8-20135毒株的交叉保护。ISA78VG&MPLA和ISA78VG&CpG&MPLA组合诱导产生更强的IFN-γ，其中CpG进一步放大了免疫反应。特别是ISA78VG&Quil-A制剂刺激了快速的抗体反应，并在第14天实现了100%的血清转化，以及针对重组HA抗原和H5N6-20053病毒的高滴度血凝抑制（HI）抗体。