Prathiksha D, Rajeshkumar S, Lingaraj J, Pradeep M
Nanobiomedicine Lab, Centre for Global Health Research, Saveetha Medical College and Hospitals, Saveetha Institute of Medical and Technical Sciences, Thandalam, Chennai, Tamil Nadu, India.
Department of Physiology, Saveetha Medical College and Hospitals, Saveetha Institute of Medical and Technical Sciences, Thandalam, Chennai, Tamil Nadu, India.
Med J Malaysia. 2025 Jan;80(Suppl 1):1-6.
Silver nanoparticles (AgNPs) are effective against almost all kinds of pathogenic organisms. The green synthesis of AgNPs utilizing extracts from medicinal plants is being researched to examine the therapeutic advantages of AgNPs because the chemical production of AgNPs is more toxic. In this study, the stem extract of Ocimum Gratissimum (OG) also known as Karunthulasi or wild basil for green synthesis of AgNPs and evaluating their antiinflammatory and antimicrobial effects.
The produced nanoparticles were characterized using UV-visible spectroscopy. The Bovine Serum Assay (BSA) and Egg Albumin (EA) assays were used to assess the anti-inflammatory effects. The protein denaturation of AgNPs was calculated and compared to a standard to determine the anti-inflammatory activity of green synthesized AgNPs. Using varying concentrations of OGmediated AgNPs in Mueller Hinton Agar (MHA), the antimicrobial effects of OG have been investigated against E. coli, S. aureus, and Pseudomonas sp. Additionally, by measuring optical density, the time-kill curve analysis for E. Coli and S. Aureus has been examined from one hour for up to five hours.
The green synthesized AgNPs were developed successfully using a plant Ocimum gratissimum. The synthesized AgNPs exhibited a maximum absorption peak at 440 nm and SEM analysis revealed that the synthesized AgNPs were spherical and oval. The result findings of the anti-inflammatory activity reveal that AgNPs have great potential when compared to the standard. At the concentration of 50 μg/mL, AgNPs exhibit 76% in BSA assay and 74% in EA assay, where the standard shows 80% inhibition. The antimicrobial activity showed a zone of inhibition around 19mm for E. coli and a 20mm zone of inhibition for S. aureus and Pseudomonas sp., which shows the efficacy of AgNPs. The time-kill assay shows that the optical density of E. coli and S. aureus was reduced to 0.1 after 5 hours of incubation, which shows the potential of green synthesized AgNPs.
OG-mediated AgNPs have both antiinflammatory and antimicrobial effects. Anti-inflammatory effects are better when compared to standard drugs. Antimicrobial effects are better for Gram-negative bacteria.
银纳米颗粒（AgNPs）对几乎所有种类的致病生物都有效。由于AgNPs的化学生产毒性更大，因此正在研究利用药用植物提取物进行AgNPs的绿色合成，以检验其治疗优势。在本研究中，使用也被称为Karunthulasi或野生罗勒的丁香罗勒（OG）的茎提取物进行AgNPs的绿色合成，并评估其抗炎和抗菌作用。
使用紫外可见光谱对制备的纳米颗粒进行表征。采用牛血清试验（BSA）和蛋清（EA）试验评估抗炎作用。计算AgNPs的蛋白质变性，并与标准品进行比较，以确定绿色合成的AgNPs的抗炎活性。在穆勒-欣顿琼脂（MHA）中使用不同浓度的OG介导的AgNPs，研究了OG对大肠杆菌、金黄色葡萄球菌和假单胞菌属的抗菌作用。此外，通过测量光密度，对大肠杆菌和金黄色葡萄球菌进行了从1小时到5小时的时间-杀菌曲线分析。
使用植物丁香罗勒成功制备了绿色合成的AgNPs。合成的AgNPs在440nm处表现出最大吸收峰，扫描电子显微镜（SEM）分析表明合成的AgNPs为球形和椭圆形。抗炎活性的结果表明，与标准品相比，AgNPs具有很大的潜力。在50μg/mL的浓度下，AgNPs在BSA试验中表现出76%的抑制率，在EA试验中表现出74%的抑制率，而标准品的抑制率为80%。抗菌活性显示，对大肠杆菌的抑菌圈约为±9mm，对金黄色葡萄球菌和假单胞菌属的抑菌圈为20mm，这表明了AgNPs的有效性。时间-杀菌试验表明，大肠杆菌和金黄色葡萄球菌在培养5小时后光密度降低到0.1，这表明了绿色合成的AgNPs的潜力。
OG介导的AgNPs具有抗炎和抗菌作用。与标准药物相比，抗炎效果更好。对革兰氏阴性菌的抗菌效果更好。
