石榴皮提取物介导的硫酸钙纳米颗粒抗炎、抗氧化活性的体外评价

In vitro evaluation of anti-inflammatory, anti-oxidant activity of pomegranate peel extract mediated calcium sulfate nano particles.

作者信息

Dathan P, Nallaswamy D, Rajeshkumar S, Joseph S, Shahin I, Tharani M

机构信息

Department of Prosthodontics, Saveetha Dental College and Hospitals, SIMATS, Chennai, India.

Nanomedicine Lab, Department of Pharmacology, Saveetha Dental College and Hospitals, SIMATS, Chennai, India.

出版信息

Med J Malaysia. 2025 Jan;80(Suppl 1):44-51.

PMID:39773942

Abstract

INTRODUCTION

Pomegranate peel is considered a reservoir of biologically active compounds, the presence of which provides anti-inflammatory and antioxidant properties to peel extracts. Calcium sulfate is considered an ideal boneaugmenting material, and in the present study, pomegranate peel extract-mediated calcium sulfate nano particles (PPE CaSo4 NPs) were synthesized and their anti-inflammatory and antioxidant properties were evaluated. This study aimed to evaluate the biological effects of PPE CaSo4 NPs, with a focus on their anti-inflammatory and antioxidant properties. The objectives were to green - synthesize PPE CaSo4 NPs, analyze their optical characteristics using UV-visible spectra analysis, and subsequently evaluate their antioxidant and anti-inflammatory activities.

MATERIALS AND METHODS

UV-visible spectra analysis was employed to investigate the optical characteristics and surface morphology, such as the size and shape of PPE CaSo4 NPs synthesized at different time intervals, which were characterized using a Scanning Electron Microscope. Anti-inflammatory activity was evaluated using bovine serum albumin denaturation assay (BSA), and egg albumin denaturation assay (EA) was compared with diclofenac sodium as a standard. Antioxidant activity was measured using 2,2 Diphenyl -1- Picryl hydraxylhydrate assay (DPPH), hydrogen peroxide radical scavenging assay (H2O2), and Ferric Reducing Antioxidant power assay (FRAP). Comparison made with ascorbic acid as the standard.

RESULTS

Anti-inflammatory activity was observed at all concentrations of PPE CaSo4 NPs, and there was no significant difference between the test material and the standard p>0.05. A significant difference was found for the antioxidant activity between PPE CaSo4 NPs and the standard in concentrations of 10 μl, for DPPH, 10 μl and 20 μl for H2O2 (p<0.05) between the concentrations of 30, 40, and 50, and there was no significant difference between the test material and the standard in all three tests conducted.

CONCLUSION

The study concluded that the PPE CaSo4 NPs have Anti-inflammatory and Antioxidant activities and are concentration-dependent.

摘要

引言

石榴皮被认为是生物活性化合物的储存库，其存在赋予了果皮提取物抗炎和抗氧化特性。硫酸钙被认为是一种理想的骨增强材料，在本研究中，合成了石榴皮提取物介导的硫酸钙纳米颗粒（PPE CaSo4 NPs），并评估了它们的抗炎和抗氧化特性。本研究旨在评估PPE CaSo4 NPs的生物学效应，重点关注其抗炎和抗氧化特性。目标是绿色合成PPE CaSo4 NPs，使用紫外可见光谱分析来分析它们的光学特性，随后评估它们的抗氧化和抗炎活性。

材料与方法

采用紫外可见光谱分析来研究光学特性和表面形态，例如在不同时间间隔合成的PPE CaSo4 NPs的大小和形状，使用扫描电子显微镜对其进行表征。使用牛血清白蛋白变性测定法（BSA）评估抗炎活性，并将卵清蛋白变性测定法（EA）与作为标准的双氯芬酸钠进行比较。使用2,2 - 二苯基 - 1 - 苦基肼自由基清除测定法（DPPH）、过氧化氢自由基清除测定法（H2O2）和铁还原抗氧化能力测定法（FRAP）测量抗氧化活性。以抗坏血酸作为标准进行比较。

结果

在所有浓度的PPE CaSo4 NPs中均观察到抗炎活性，测试材料与标准之间无显著差异（p>0.05）。在浓度为10 μl时，PPE CaSo4 NPs与标准之间的抗氧化活性存在显著差异，对于DPPH，在30、40和50浓度之间，H2O2在10 μl和20 μl时（p<0.05），并且在所有三项测试中测试材料与标准之间均无显著差异。