Rani Jyoti, Singh Sushila, Beniwal Anuradha, Kakkar Simran, Moond Monika, Sangwan Seema, Kumari Sachin
Department of Chemistry, CCS Haryana Agricultural University, Hisar, 125004, India.
Department of Microbiology, CCS Haryana Agricultural University, Hisar, 125004, India.
Discov Nano. 2024 Oct 2;19(1):160. doi: 10.1186/s11671-024-04103-8.
Biologically produced silver nanoparticles are becoming a more appealing option than chemically produced antioxidants and antimicrobial agents, because they are safer, easier to manufacture and have medicinal properties at lower concentrations. In this work, we employed the aqueous pomegranate peel extract (PPE) to synthesize silver nanoparticles (PPE-AgNPs), as peel extract is a rich source of phytochemicals which functions as reducing agent for the synthesis of PPE-AgNPs. Additionally, the PPE was examined quantitatively for total phenolics and total flavonoids content. PPE-AgNPs were characterized using analytical techniques including UV-Vis spectroscopy, DLS, FTIR, XRD, HRTEM and FESEM, evaluated in vitro against the plant pathogenic microbes and also for antioxidant activities. Analytical techniques (HRTEM and FESEM) confirmed the spherical shape and XRD technique revealed the crystalline nature of synthesized PPE-AgNPs. Quantitative analysis revealed the presence of total phenolics (269.93 ± 1.01 mg GAE/g) and total flavonoids (119.70 ± 0.83 mg CE/g). Biosynthesized PPE-AgNPs exhibited significant antibacterial activity against Klebsiella aerogenes and Xanthomonas axonopodis, antifungal activity against Colletotrichum graminicola and Colletotrichum gloesporioides at 50 µg/mL concentration. The antioxidant potential of biosynthesized PPE-AgNPs was analysed via ABTS (IC 4.25 µg/mL), DPPH (IC 5.22 µg/mL), total antioxidant (86.68 g AAE/mL at 10 µg/mL) and FRAP (1.93 mM Fe(II)/mL at 10 µg/mL) assays. Cytotoxicity of PPE-AgNPs was valuated using MTT assay and cell viability of 83.32% was determined at 100 µg/mL concentration. These investigations suggest that synthesized PPE-AgNPs might prove useful for agricultural and medicinal purposes in the future.
生物合成的银纳米颗粒正成为比化学合成的抗氧化剂和抗菌剂更具吸引力的选择，因为它们更安全、易于制造且在较低浓度下就具有药用特性。在本研究中，我们利用石榴皮水提取物（PPE）合成银纳米颗粒（PPE-AgNPs），因为皮提取物富含植物化学物质，可作为合成PPE-AgNPs的还原剂。此外，还对PPE中的总酚和总黄酮含量进行了定量检测。使用紫外可见光谱、动态光散射、傅里叶变换红外光谱、X射线衍射、高分辨率透射电子显微镜和场发射扫描电子显微镜等分析技术对PPE-AgNPs进行了表征，并对其进行了体外抗植物病原微生物活性和抗氧化活性评估。分析技术（高分辨率透射电子显微镜和场发射扫描电子显微镜）证实了合成的PPE-AgNPs为球形，X射线衍射技术揭示了其晶体性质。定量分析表明，总酚含量为（269.93±1.01mg没食子酸当量/g），总黄酮含量为（119.70±0.83mg儿茶素当量/g）。生物合成的PPE-AgNPs在50μg/mL浓度下对产气克雷伯菌和野油菜黄单胞菌表现出显著的抗菌活性，对禾谷炭疽菌和胶孢炭疽菌表现出抗真菌活性。通过ABTS（IC 4.25μg/mL）、DPPH（IC 5.22μg/mL）、总抗氧化能力（10μg/mL时为86.68g抗坏血酸当量/mL）和铁还原抗氧化能力（10μg/mL时为1.93mM Fe(II)/mL）测定分析了生物合成的PPE-AgNPs的抗氧化潜力。使用MTT法评估了PPE-AgNPs的细胞毒性，在100μg/mL浓度下测定细胞活力为83.32%。这些研究表明，合成的PPE-AgNPs未来可能在农业和医学领域具有应用价值。
