Appleyard Sharon A, Ward Robert D, Pogonoski John J, Graham Alastair, Last Peter R, Deagle Bruce E, Holmes Bronwyn, Gomon Martin F, Bray Dianne J, Johnson Jeffrey W, Hay Amanda C, Moore Glenn I, Hammer Michael P, Russell Barry, Graham Ken J
CSIRO Australian National Fish Collection, National Research Collections Australia, Hobart, TAS, 7000, Australia.
CSIRO Environment, Hobart, TAS, 7000, Australia.
Sci Data. 2025 Jan 7;12(1):21. doi: 10.1038/s41597-025-04375-4.
Over 15 000 species of fishes are found globally in the marine environment and DNA barcodes are used extensively to describe, catalogue, understand and manage this diversity. The dataset outlined here represents a DNA barcode reference library of the mitochondrial cytochrome c oxidase subunit 1 gene (COI) from 9767 voucher specimens (representing at least 2220 species and 288 families) of marine fishes. This publicly available dataset in the Barcode of Life Data System (BOLD) represents 17 years (2005-2022) of barcoding of marine fishes identified from Australian territorial waters. Tissues targeted for sequencing with their matching physical specimens (and extracted DNA), obtained via a multi-agency sampling effort, are mostly maintained and curated by the CSIRO Australian National Fish Collection (ANFC) in Hobart, Australia. Species-level integrated taxonomy (assigned after combined morphological and genetic assessment) has been determined for 91% of the dataset. The library represents the most complete COI barcode reference dataset for marine fishes from Australian waters and is currently utilised for integrated taxonomy, (meta)barcoding and eDNA studies.
全球海洋环境中发现了超过15000种鱼类，DNA条形码被广泛用于描述、编目、理解和管理这种多样性。此处概述的数据集代表了来自9767个凭证标本（代表至少2220个物种和288个科）的海洋鱼类线粒体细胞色素c氧化酶亚基1基因（COI）的DNA条形码参考文库。生命条形码数据系统（BOLD）中的这个公开数据集代表了17年（2005 - 2022年）对从澳大利亚领海鉴定出的海洋鱼类进行条形码分析的结果。通过多机构采样获得的用于测序的组织及其匹配的实物标本（和提取的DNA），大多由位于澳大利亚霍巴特的联邦科学与工业研究组织澳大利亚国家鱼类标本馆（ANFC）保存和管理。已对91%的数据集确定了物种水平的综合分类法（在形态学和遗传学综合评估后确定）。该文库是澳大利亚海域海洋鱼类最完整的COI条形码参考数据集，目前用于综合分类学、（元）条形码分析和环境DNA研究。
