Shates Tessa M, Realegeno Susan, Prince Harry E, Marlowe Elizabeth M
Quest Diagnostics Inc., San Juan Capistrano, CA.
Sex Transm Dis. 2025 Feb 1;52(2):125-128. doi: 10.1097/OLQ.0000000000002087.
Approximately 2% of HerpeSelect herpes simples virus type 2 (HSV-2) IgG enzyme immunoassay (screen assay) sera-positive samples do not confirm using an HSV-2 IgG inhibition assay. Of these, roughly 1.33% are confirmed negative, and a small proportion (0.2%) are indeterminate due to inhibition observed with HSV-1 and HSV-2 lysate. The objective of this study was to assess the relationship between HSV-1 IgG serostatus and HSV-2 IgG confirmation results using the HSV-2 IgG inhibition assay.
This study included consecutive HSV-2 IgG screen-positive specimens regardless of index value. Specimens were also tested for HSV-1 IgG. The HSV-2 IgG confirmation results from the inhibition assay were evaluated to assess the relationship between inhibition results and HSV-1 serostatus.
Of the 21,006 positive HSV-2 IgG specimens, 13,237 (63%) were also positive for HSV-1 IgG. The proportion of HSV-1 IgG-positive samples among inhibition-indeterminate samples (33 to 40 [83%]) was higher than among inhibition-negative (167 of 279 [60%]) and inhibition-positive (13,041 of 20,691 [63%]) samples. Among HSV-1 IgG-positive samples, the median HSV-1 IgG index was also significantly higher for the inhibition-indeterminate group (median, 48.8) than for the inhibition-negative (median, 30.7) and inhibition-positive groups (median, 31.2; P = 0.00013 and P = 3.1e - 05, respectively).
These findings suggest that HSV-2 IgG inhibition-indeterminate, but not inhibition-negative, results are associated with higher index values of HSV-1 IgG. Further studies are needed to understand other factors associated with inhibition-indeterminate results.
在使用HerpeSelect 2型单纯疱疹病毒（HSV - 2）IgG酶免疫测定法（筛查测定）检测血清呈阳性的样本中，约2%的样本无法通过HSV - 2 IgG抑制测定法得到确认。其中，约1.33%被确认为阴性，还有一小部分（0.2%）因被HSV - 1和HSV - 2裂解物抑制而结果不确定。本研究的目的是使用HSV - 2 IgG抑制测定法评估HSV - 1 IgG血清学状态与HSV - 2 IgG确认结果之间的关系。
本研究纳入了连续的HSV - 2 IgG筛查呈阳性的标本，无论其指数值如何。标本还进行了HSV - 1 IgG检测。对抑制测定法得到的HSV - 2 IgG确认结果进行评估，以评估抑制结果与HSV - 1血清学状态之间的关系。
在21,006份HSV - 2 IgG阳性标本中，13,237份（63%）HSV - 1 IgG也呈阳性。抑制结果不确定的样本中HSV - 1 IgG阳性样本的比例（33至40份中的83%）高于抑制阴性样本（279份中的167份，60%）和抑制阳性样本（20,691份中的13,041份，63%）。在HSV - 1 IgG阳性样本中，抑制结果不确定组的HSV - 1 IgG指数中位数（中位数为48.8）也显著高于抑制阴性组（中位数为30.7）和抑制阳性组（中位数为31.2；P分别为0.00013和3.1×10⁻⁵）。
这些发现表明，HSV - 2 IgG抑制结果不确定而非抑制阴性，与HSV - 1 IgG较高的指数值相关。需要进一步研究以了解与抑制结果不确定相关的其他因素。
