评估一种新型的血清学算法来检测单纯疱疹病毒 1 型或 2 型抗体。

Evaluation of a novel serology algorithm to detect herpes simplex virus 1 or 2 antibodies.

机构信息

Provincial Laboratory for Public Health, Edmonton, Alberta, Canada.

出版信息

Sex Transm Dis. 2010 Nov;37(11):696-9. doi: 10.1097/OLQ.0b013e3181e2cdab.

DOI:10.1097/OLQ.0b013e3181e2cdab

PMID:20693937

Abstract

BACKGROUND

Herpes simplex virus (HSV)-type specific serology (TSS) testing has been commercially available for nearly a decade. Guidelines on appropriate use of such testing exist, including Canadian-based recommendations. Despite this, most Canadian laboratories do not offer HSV-type specific serology and many provide only nontype-specific HSV serology tests.

METHODS

At the Alberta Provincial Laboratory, HSV TSS is performed using the following algorithm (termed the Alberta algorithm). Eligible specimens are first tested with a nontype-specific kit (Behring Enzygnost IgG). If positive, sera are then tested using the Focus HerpeSelect-2 assay to establish HSV-2 infection. If the HerpeSelect-2 result is negative, the result is reported as anti-HSV-1 positive. In this study we sought a validation of the Alberta algorithm by testing 344 serum samples with the Behring Enzygnost IgG assay, the type specific Focus HerpeSelect 1 and 2 assays, and by Western blot (WB).

RESULTS

Taking the WB as a gold standard, the Behring Enzygnost IgG assay showed a sensitivity of 94% and a specificity of 100%, whereas the Alberta algorithm had a sensitivity of 92% and a specificity of 97% for the detection of HSV-2 antibodies, and a sensitivity of 94% and a specificity of 100% for the detection of HSV-1 antibodies in HSV-2 negative sera. Focus HerpeSelect 1 and HerpeSelect 2 sensitivities against WB were 88% and 91%, whereas specificities were 95% and 97% for HSV-1 and HSV-2, respectively.

CONCLUSIONS

The Alberta algorithm was at least equivalent to HerpeSelect 1 and 2 in detecting anti-HSV antibodies. Although sensitivity and specificity were higher, the differences were not statistically significant.

摘要

背景

单纯疱疹病毒（HSV）型特异性血清学（TSS）检测已商业化近十年。存在关于此类检测的使用的指南，包括基于加拿大的建议。尽管如此，大多数加拿大实验室不提供 HSV 型特异性血清学，许多实验室仅提供非型特异性 HSV 血清学检测。

方法

在艾伯塔省省级实验室，使用以下算法（称为艾伯塔算法）进行 HSV TSS。首先使用非型特异性试剂盒（贝林 Enzygnost IgG）对合格标本进行检测。如果阳性，然后使用 Focus HerpeSelect-2 测定法检测血清以确定 HSV-2 感染。如果 HerpeSelect-2 结果为阴性，则报告结果为抗 HSV-1 阳性。在这项研究中，我们通过使用 Behring Enzygnost IgG 测定法、型特异性 Focus HerpeSelect 1 和 2 测定法以及 Western blot（WB）检测了 344 份血清样本，对艾伯塔算法进行了验证。

结果

以 WB 作为金标准，贝林 Enzygnost IgG 检测法对 HSV-2 抗体的敏感性为 94%，特异性为 100%，而艾伯塔算法对 HSV-2 抗体的敏感性为 92%，特异性为 97%，对 HSV-1 抗体的敏感性为 94%，特异性为 100%，对 HSV-2 阴性血清的 HSV-1 抗体的敏感性为 94%，特异性为 100%。Focus HerpeSelect 1 和 HerpeSelect 2 对 WB 的敏感性分别为 88%和 91%，而特异性分别为 95%和 97%，用于检测 HSV-1 和 HSV-2。