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人牙髓干细胞来源外泌体对人成纤维细胞生物学行为的影响

Effects of exosomes from human dental pulp stem cells on the biological behavior of human fibroblasts.

作者信息

Chen Guan-Yu, Fu Ling-Ling, Ye Hui-Ping, Cheng Ping, Feng Hong-Chao, Yan Ming

机构信息

College of Stomatology, Guizhou Medical University, Guiyang, 550000, China.

Department of Oral and Maxillofacial Surgery, Guiyang Hospital of Stomatology, Guiyang, 550000, China.

出版信息

Sci Rep. 2025 Jan 7;15(1):1134. doi: 10.1038/s41598-024-78388-1.

DOI:10.1038/s41598-024-78388-1
PMID:39774130
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11707004/
Abstract

The aim of this study was to investigate the effect of dental pulp stem cell-derived exosomes (DPSCs-Exos) on the biological behaviour of fibroblasts, particularly on keloid fibroblasts (KFs) and normal skin fibroblasts (NFs), with a view to providing new insights into cellular regenerative medicine. We obtained DPSCs-Exos by ultracentrifugation and co-cultured it with KFs and NFs. We detected its effect on cell proliferation using the CCK-8 assay; cell migration ability by cell scratch and Transwell assays; extracellular matrix synthesis using the hydroxyproline content assay; the expression levels of genes associated with fibrosis by PCR assay; and the expression levels of proteins related to fibrosis in the cells using the Western Blot method. DPSCs-Exos was able to be taken up by fibroblasts after addition to the culture medium and affected the biological behavior of NFs and KFs. DPSCs-Exos promoted the proliferation of NFs, inhibited the migration and extracellular matrix synthesis of KFs. In addition, DPSCs-Exos was able to inhibit the expression of fibrosis-related genes and proteins in KFs. This study highlights the role of DPSCs-Exos in regulating the biological behaviour of fibroblasts, providing new insights for future applications in the field of cell-free regenerative medicine.

摘要

本研究的目的是探讨牙髓干细胞来源的外泌体（DPSCs-Exos）对成纤维细胞生物学行为的影响，特别是对瘢痕疙瘩成纤维细胞（KFs）和正常皮肤成纤维细胞（NFs）的影响，以期为细胞再生医学提供新的见解。我们通过超速离心获得DPSCs-Exos，并将其与KFs和NFs共培养。我们使用CCK-8法检测其对细胞增殖的影响；通过细胞划痕和Transwell法检测细胞迁移能力；使用羟脯氨酸含量测定法检测细胞外基质合成；通过PCR法检测与纤维化相关基因的表达水平；并使用蛋白质印迹法检测细胞中与纤维化相关蛋白质的表达水平。将DPSCs-Exos添加到培养基中后，能够被成纤维细胞摄取，并影响NFs和KFs的生物学行为。DPSCs-Exos促进NFs的增殖，抑制KFs的迁移和细胞外基质合成。此外，DPSCs-Exos能够抑制KFs中与纤维化相关基因和蛋白质的表达。本研究突出了DPSCs-Exos在调节成纤维细胞生物学行为中的作用，为未来在无细胞再生医学领域的应用提供了新的见解。

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本文引用的文献

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Crosstalk between fibroblasts and immunocytes in fibrosis: From molecular mechanisms to clinical trials.成纤维细胞与免疫细胞在纤维化中的相互作用：从分子机制到临床试验。
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Therapeutic role of exosomes and conditioned medium in keloid and hypertrophic scar and possible mechanisms.
外泌体和条件培养基在瘢痕疙瘩和增生性瘢痕中的治疗作用及可能机制。
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Combination of Fractional Er:YAG Laser, Pulsed Dye Laser, and Intralesional Triamcinolone With 5-Fluorouracil for Keloid Treatment.剥脱性铒激光、脉冲染料激光及曲安奈德联合5-氟尿嘧啶治疗瘢痕疙瘩
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Fibroblasts - the cellular choreographers of wound healing.成纤维细胞——伤口愈合的细胞“编舞家”。
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Human dental pulp stem cells (hDPSCs) promote the lipofibroblast transition in the early stage of a fibro-inflammatory process.人牙髓干细胞（hDPSCs）在纤维炎症过程的早期促进脂肪成纤维细胞转变。
Front Cell Dev Biol. 2023 May 3;11:1196023. doi: 10.3389/fcell.2023.1196023. eCollection 2023.
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Revealing the roles of glycosphingolipid metabolism pathway in the development of keloid: a conjoint analysis of single-cell and machine learning.揭示糖脂代谢途径在瘢痕疙瘩发展中的作用：单细胞和机器学习的联合分析。
Front Immunol. 2023 Apr 24;14:1139775. doi: 10.3389/fimmu.2023.1139775. eCollection 2023.
8
Adipose-derived mesenchymal stem cells-sourced exosomal microRNA-7846-3p suppresses proliferation and pro-angiogenic role of keloid fibroblasts by suppressing neuropilin 2.脂肪间充质干细胞来源的外泌体 microRNA-7846-3p 通过抑制神经纤毛蛋白 2 抑制瘢痕疙瘩成纤维细胞的增殖和促血管生成作用。
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JCI Insight. 2023 Mar 22;8(6):e157091. doi: 10.1172/jci.insight.157091.