Usman Muhammad, Rizwan Hafiz Muhammad, Sajid Muhammad Sohail, Kausar Razia, Tahir Urfa Bin, Abbas Haider, Ateeq Muhammad Khalil, Raza Mohsin, Maqbool Mahvish, Fouad Dalia, Ataya Farid S
Section of Histology, Department of Basic Sciences, KBCMA College of Veterinary and Animal Sciences, Narowal, Sub-campus UVAS, Lahore, Pakistan.
Section of Parasitology, Department of Pathobiology, KBCMA College of Veterinary and Animal Sciences, Narowal, Sub-campus UVAS, Lahore, Pakistan.
Rev Bras Parasitol Vet. 2024 Dec 20;33(4):e016824. doi: 10.1590/S1984-29612024080. eCollection 2024.
A total of 384 animals (sheep, goat, cattle, and buffalo) were examined for the presence of hydatid cysts only in the lungs. The lung tissue samples associated with the hydatid cyst were collected immediately after slaughter, followed by fixation in 10% formalin. The fixed tissue was subjected to paraffin embedding technique. Tissue sections of 5 microns were cut by microtome and stained using Harri's Haematoxilin and Eosin method. Overall, 13.80% of ruminants were found positive for lung infections with hydatid cyst. Only the sex of ruminants showed significant (P < 0.05) association with the infection of hydatid cyst in lungs. All other variables, such as species of ruminants, age, and months showed non-significant (P > 0.05) association. Pulmonary sections taken from infected animals revealed laminated membranes encased in a region with significant (P < 0.05) cellular infiltration (53.4 ± 7.9 µm2), primarily composed of lymphocytes, plasma cells, macrophages, and occasionally neutrophils, and eosinophils. In addition, significant (P < 0.05) epithelial disruption in the bronchioles (0.94 ± 0.05 µm2) and alveolar septa were also noticed in sections. These histopathological findings lead to the conclusion that pathological changes occur in the tissues surrounding the cyst as well as in areas more distant from the cyst.
总共对384只动物（绵羊、山羊、牛和水牛）进行了检查，仅查看其肺部是否存在包虫囊肿。与包虫囊肿相关的肺组织样本在屠宰后立即采集，随后用10%福尔马林固定。固定后的组织进行石蜡包埋技术处理。用切片机切成5微米厚的组织切片，并用哈里苏木精和伊红方法染色。总体而言，发现13.80%的反刍动物肺部感染包虫囊肿呈阳性。只有反刍动物的性别与肺部包虫囊肿感染呈显著（P<0.05）关联。所有其他变量，如反刍动物的种类、年龄和月份，均无显著（P>0.05）关联。取自感染动物的肺部切片显示，在一个有显著（P<0.05）细胞浸润（53.4±7.9平方微米）的区域内有分层膜，主要由淋巴细胞、浆细胞、巨噬细胞组成，偶尔还有中性粒细胞和嗜酸性粒细胞。此外，在切片中还注意到细支气管（0.94±0.05平方微米）和肺泡间隔有显著（P<0.05）的上皮破坏。这些组织病理学发现得出结论，囊肿周围组织以及离囊肿较远的区域都会发生病理变化。
