Sol Izabella, Hadad Henrique, Kano Tatsuya Henrique, Tonini Karen Rawen, Lage Nunes Martina Andreia, Ponzoni Daniela
Department of Diagnosis and Surgery, São Paulo State University (UNESP), School of Dentistry, Araçatuba, São Paulo, Brazil.
PLoS One. 2024 Dec 30;19(12):e0312280. doi: 10.1371/journal.pone.0312280. eCollection 2024.
Rotatory devices are essential in clinical surgical practice, however, depending on the different systems available, their function can impact bone repair and postoperative responses on varying scales. This impact underscores the need to explore new techniques aiming to enhance bone repair. This study aimed to assess the immediate and delayed effects on bone healing in subcritical bone defects using both air turbine and an electric handpiece. For this purpose, 40 male Wistar rats were allocated into two groups. The Control Group (CG) had bone defect made using an air turbine device, while the Experimental Group (EG) had defects made using an electric handpiece. Ten animals were sacrificed for each time of evaluation. Bone neoformation, microstructure, and collagen organization were assessed ate 7, 15 and 30 days postoperative. Inflammatory profiling was conducted at 7 and 15 days. Immediate thermal osteonecrosis were evaluated after the use of rotary systems. Multivariate analysis was used to access statistical differences. The EG exhibited enhanced parameters of bone neoformation in all analyses, with statistical difference between 15 and 30 days (P = .0002) and in comparison with CG in 30 days (P = .0009). A reduced number of inflammatory cells and increased angiogenesis in the initial periods was seen in EG, corroborating the consistent values of collagen type 1 and a decrease of collagen type 3 over times. Immediate thermal osteonecrosis was statistically higher for the CG (P < .05), which showed adequate neoformation of subcritical defects but consistently lower values than those found in the EG. These data suggest that the electric handpiece demonstrated more bone repair area, proving to be an excellent alternative to surgical practice.
旋转设备在临床外科实践中至关重要，然而，根据现有的不同系统，其功能会在不同程度上影响骨修复和术后反应。这种影响凸显了探索旨在增强骨修复的新技术的必要性。本研究旨在评估使用气涡轮和电动手机对亚临界骨缺损骨愈合的即时和延迟影响。为此，将40只雄性Wistar大鼠分为两组。对照组（CG）使用气涡轮设备制造骨缺损，而实验组（EG）使用电动手机制造缺损。每次评估时处死10只动物。在术后7天、15天和30天评估骨新生、微观结构和胶原组织。在7天和15天进行炎症分析。在使用旋转系统后评估即时热骨坏死。采用多变量分析来确定统计学差异。在所有分析中，EG组的骨新生参数均有所增强，在15天和30天之间存在统计学差异（P = .0002），与30天时的CG组相比也有统计学差异（P = .0009）。在EG组中，初期炎症细胞数量减少，血管生成增加，这与I型胶原的持续值以及随着时间推移III型胶原的减少相一致。CG组的即时热骨坏死在统计学上更高（P < .05），该组亚临界缺损有足够的新生，但始终低于EG组的值。这些数据表明，电动手机显示出更多的骨修复区域，被证明是外科实践的一个极佳选择。
