Cavalcante Gurgiane Rodrigues Gurgel, Moreno Mariana Cabral, Pirih Flavia Queiroz, Soares Vanessa de Paula, Silveira Éricka Janine Dantas da, Silva José Sandro Pereira da, Pereira Hallissa Simplício Gomes, Klein Katherine Pennington, Lopes Maria Luiza Diniz de Sousa, Araujo Aurigena Antunes de, Martins Agnes Andrade, Lins Ruthinéia Diógenes Alves Uchôa
Universidade Federal do Rio Grande do Norte - UFRN, Graduate Program in Dental Sciences, Department of Dentistry, Natal, Brazil.
University of California- Ucla, School of Dentistry, Los Angeles, CA, USA.
Braz Oral Res. 2024 Dec 20;38:e131. doi: 10.1590/1807-3107bor-2024.vol38.0131. eCollection 2024.
The aim of this study was to investigate the effect of thermogenic supplementation on the bone tissue of rats subjected to orthodontic movement. A total of 38 male Wistar rats underwent orthodontic movement of the left permanent maxillary first molars for 21 days. The rats were assigned to three groups: Control group: water; Thermogenic 1: C4 Beta Pump thermogenic; or Thermogenic 2: PRE-HD/Pre-workout. Micro-computed tomography (micro-CT) was used to investigate the dynamic changes in the microstructure of alveolar bone during orthodontic tooth movement in rats. Histopathologic analysis was performed by hematoxylin and eosin (H&E) staining, whereas tartrate-resistant acid phosphatase (TRAP) was employed for osteoclast count. Maxillary tissue was collected and evaluated by immunohistochemistry for receptor activator of NF-κB (RANK), receptor activator of NF-κB ligand (RANKL), and osteoprotegerin (OPG). The Thermogenic 2 group exhibited a significantly lower percentage of bone volume fraction (BV/TV) (68.21% ± 17.70%) compared to the control (86.84% + 12.91%) and Thermogenic 1 groups (86.84% + 15.94%) (p < 0.05). The control group had a significantly higher mean orthodontic movement in the mesial direction (0.2143 mm + 0.1513 mm) than the Thermogenic 2 group (0.0420 mm + 0.05215 mm) (p < 0.05). The Thermogenic 2 and Thermogenic 1 groups showed a stronger immunostaining for RANKL when compared to the control group (p < 0.05). The supplementation used in the Thermogenic 2 group (PRE-HD/Pre-workout) induced alveolar bone loss in rats subjected to orthodontic movement, which can be related to the regulation of the RANK/RANKL/OPG signaling pathway. This suggests the influence of thermogenic supplements on bone metabolism seems to depend on their composition.
本研究的目的是调查产热补充剂对接受正畸移动的大鼠骨组织的影响。总共38只雄性Wistar大鼠的左侧上颌第一恒磨牙进行正畸移动21天。将大鼠分为三组：对照组：水；产热1组：C4 Beta Pump产热剂；或产热2组：PRE-HD/运动前补充剂。使用微型计算机断层扫描（micro-CT）研究大鼠正畸牙齿移动过程中牙槽骨微观结构的动态变化。通过苏木精和伊红（H&E）染色进行组织病理学分析，而抗酒石酸酸性磷酸酶（TRAP）用于破骨细胞计数。收集上颌组织并通过免疫组织化学评估核因子κB受体激活剂（RANK）、核因子κB受体激活剂配体（RANKL）和骨保护素（OPG）。与对照组（86.84% + 12.91%）和产热1组（86.84% + 15.94%）相比，产热2组的骨体积分数（BV/TV）百分比显著降低（68.21% ± 17.70%）（p < 0.05）。对照组在近中方向的平均正畸移动（0.2143 mm + 0.1513 mm）显著高于产热2组（0.0420 mm + 0.05215 mm）（p < 0.05）。与对照组相比，产热2组和产热1组对RANKL的免疫染色更强（p < 0.05）。产热2组使用的补充剂（PRE-HD/运动前补充剂）在接受正畸移动的大鼠中诱导牙槽骨丢失，这可能与RANK/RANKL/OPG信号通路的调节有关。这表明产热补充剂对骨代谢的影响似乎取决于它们的成分。
