Department of Advanced General Dentistry, Yonsei University College of Dentistry, Seoul, Korea.
Human Identification Research Institute, Yonsei University College of Dentistry, Seoul, Korea.
Prog Orthod. 2024 Nov 25;25(1):45. doi: 10.1186/s40510-024-00544-0.
This study aimed to evaluate the effect of systemically administered anti-sclerostin antibodies (Anti-Scl Ab) on orthodontic tooth movements (OTM) in an ovariectomized rat.
Twenty-four 12-week-old female Sprague-Dawley rats were randomly divided into two groups: (1) ovariectomy (OVX) group, (2) ovariectomy + romosozumab (ROMO) group. OTM was performed 8 weeks after OVX. The ROMO group received subcutaneous injections of romosozumab twice a week, starting two weeks after OVX. Eight weeks after the OVX, an orthodontic force of 50 g was measured and applied by connecting orthodontic elastic bands between the maxillary first molar and a mini-screw to facilitate tooth movement (orthodontic treatment). Subsequently, the three rats were sacrificed on days 5, 7, 10, and 14. The plaster models were scanned to measure the amount of tooth movement. The effects on alveolar bone and periodontal tissues were evaluated through micro-computed tomography (micro-CT) analysis, tartrate-resistant acid phosphatase (TRAP) staining, and immunohistochemistry (IHC) analysis.
The ROMO group showed more tooth movement on day 7 of orthodontic treatment. Conversely, on days 10 and 14, relatively less movement was observed. Analysis of the root furcation area of the maxillary first molars revealed that from the 7th day, BV/TV, Tb.N., Tb.Th. increased, while Tb.Sp. decreased in the ROMO group. More TRAP-positive cells were observed in the compression side of the OVX group, the ROMO group exhibited a marked decrease in the positive expression of the RANKL, OPG, and sclerostin. The OPG/RANKL ratio showed significant differences in expression between the two groups. The ROMO group exhibited a higher OPG/RANKL ratio than the OVX group, and the tension side exhibited a higher OPG/RANKL ratio demonstrating significant differences.
Romosozumab initially accelerated tooth movements, but later decreased tooth movement. As new alveolar bone is formed, the micro-CT parameters are also improved. Osteoclasts, RANKL, OPG, and sclerostin decreased, while the OPG/RANKL ratio became higher.
本研究旨在评估全身性给予抗硬骨素抗体（Anti-Scl Ab）对去卵巢大鼠正畸牙齿移动（OTM）的影响。
将 24 只 12 周龄雌性 Sprague-Dawley 大鼠随机分为两组：（1）卵巢切除术（OVX）组，（2）卵巢切除术+Romosozumab（ROMO）组。OVX 后 8 周进行 OTM。ROMO 组在 OVX 后两周开始每周两次皮下注射 Romosozumab。OVX 后 8 周，通过在上颌第一磨牙和微型螺钉之间连接正畸弹性带施加 50 g 的正畸力，以促进牙齿移动（正畸治疗）。随后，在第 5、7、10 和 14 天处死 3 只大鼠。扫描石膏模型以测量牙齿移动量。通过微计算机断层扫描（micro-CT）分析、抗酒石酸酸性磷酸酶（TRAP）染色和免疫组织化学（IHC）分析评估对牙槽骨和牙周组织的影响。
ROMO 组在正畸治疗第 7 天牙齿移动量较大。相反，在第 10 和 14 天，观察到的移动量相对较少。对上颌第一磨牙根分叉区的分析表明，从第 7 天开始，BV/TV、Tb.N.、Tb.Th.增加，而 Tb.Sp.减少。OVX 组受压侧观察到更多的 TRAP 阳性细胞，ROMO 组 RANKL、OPG 和硬骨素的阳性表达明显减少。OPG/RANKL 比值在两组之间表达差异有统计学意义。ROMO 组的 OPG/RANKL 比值高于 OVX 组，张力侧的 OPG/RANKL 比值较高，差异有统计学意义。
Romosozumab 最初加速了牙齿移动，但后来减少了牙齿移动。随着新牙槽骨的形成，微 CT 参数也得到改善。破骨细胞、RANKL、OPG 和硬骨素减少，而 OPG/RANKL 比值升高。
