Wang Chien-Hsing, Huang Zih-Ting, Tai Kuo-Feng
Department of Surgery, Hualien Tzu Chi Hospital, Hualien, Taiwan.
School of Medicine, Tzu Chi University, Hualien, Taiwan.
PLoS One. 2025 Jan 8;20(1):e0311037. doi: 10.1371/journal.pone.0311037. eCollection 2025.
Ulva lactuca (U. lactuca) is an important seaweed species. Some ingredients in this species are thought to accelerate wound healing. However, limited data on the use of seaweed for wound healing exists. This study examined whether ethanol or aqueous extracts of U. lactuca promote antioxidant and anti-inflammatory properties in vitro and wound healing in vitro and in vivo. Cell proliferation, antioxidation, and migration were observed in NIH3T3 cells treated with U. lactuca extract in vitro. Both U. lactuca extracts were examined for their ability to inhibit inflammatory cytokine synthesis in lipopolysaccharide (LPS)-stimulated RAW 264.7 cells. In vivo experiments involved four groups of albino mice (BALB/c; 10 mice per group). One 1.0 cm2 wound was created via excision of full-thickness skin on the back of all mice. Group I mice were treated topically with the ethanol extract of U. lactuca (25 mg/mL) for 10 d. Group II mice were treated topically with an aqueous extract of U. lactuca (12.5 mg/mL) for 10 d. Group III mice received topical application of phosphate-buffered saline solution. Group IV mice wounds were maintained without treatment. Both extracts considerably increased fibroblast proliferation. The antioxidant activity of the U. lactuca extract was determined using a total antioxidant capacity assay. Both extracts inhibited the release of tumor necrosis factor-α (TNF-α) and interferon-γ (IFN-γ) from LPS-mediated inflammation in RAW 264.7 cells. These extracts also upregulated the expression of Th2 cytokines such as transforming growth factor beta 1 (TGF-β1) and interleukin 10 (IL-10) in RAW 264.7 cells under pro-inflammatory conditions. Both extracts enhanced the migratory ability of NIH3T3 cells. U. lactuca ethanol extract enhances wound healing properties in vivo. These results suggest that bioactive compounds derived from U. lactuca extract are beneficial for wound healing and anti-inflammatory therapies.
石莼是一种重要的海藻物种。该物种中的某些成分被认为可加速伤口愈合。然而，关于海藻用于伤口愈合的相关数据有限。本研究检测了石莼的乙醇提取物或水提取物是否能在体外促进抗氧化和抗炎特性，以及在体外和体内促进伤口愈合。体外观察了用石莼提取物处理的NIH3T3细胞的细胞增殖、抗氧化和迁移情况。检测了两种石莼提取物抑制脂多糖（LPS）刺激的RAW 264.7细胞中炎性细胞因子合成的能力。体内实验涉及四组白化小鼠（BALB/c；每组10只小鼠）。通过切除所有小鼠背部的全层皮肤形成一个1.0平方厘米的伤口。第一组小鼠局部用石莼乙醇提取物（25毫克/毫升）处理10天。第二组小鼠局部用石莼水提取物（12.5毫克/毫升）处理10天。第三组小鼠局部应用磷酸盐缓冲盐水溶液。第四组小鼠的伤口不做处理。两种提取物均显著增加了成纤维细胞增殖。使用总抗氧化能力测定法测定石莼提取物的抗氧化活性。两种提取物均抑制了RAW 264.7细胞中LPS介导的炎症反应中肿瘤坏死因子-α（TNF-α）和干扰素-γ（IFN-γ）的释放。在促炎条件下，这些提取物还上调了RAW 264.7细胞中Th2细胞因子如转化生长因子β1（TGF-β1）和白细胞介素10（IL-10）的表达。两种提取物均增强了NIH3T3细胞的迁移能力。石莼乙醇提取物在体内增强了伤口愈合特性。这些结果表明，石莼提取物中的生物活性化合物有利于伤口愈合和抗炎治疗。
