Tomaszewska Ewa, Dobrowolski Piotr, Dajnowska Aleksandra, Arbatowska Liwia, Puzio Iwona, Rudyk Halyna, Brezvyn Oksana, Kotsyumbas Ihor, Donaldson Janine, Śliwa Jadwiga, Arciszewski Marcin B, Muszyński Siemowit
Department of Animal Physiology, Lublin, Poland.
Department of Functional Anatomy and Cytobiology, Faculty of Biology and Biotechnology, Maria Curie-Sklodowska University, 20-033 Lublin, Poland.
J Vet Res. 2024 Oct 9;68(4):633-642. doi: 10.2478/jvetres-2024-0056. eCollection 2024 Dec.
This study explored the effects of prenatal exposure to fumonisins B (FB) on bone innervation in newborn Wistar rats.
Pregnant dams (n = 6 per group) were assigned to either the control or one of two FB-exposed groups (60 mg or 90 mg/kg body weight) from the 7 day of gestation until parturition. On the day of parturition, one male pup from each litter (n = 6 per group) was randomly selected and euthanised, and their femurs were dissected for analysis. Bone innervation was quantified by examining the morphology patterns of sympathetic, parasympathetic, sensory and cocaine- and amphetamine-regulated transcript (CART)-positive fibres. Prepared bone sections were analysed using immunohistochemistry staining for protein gene product 9.5, tyrosine hydroxylase, choline acetyltransferase, vasoactive intestinal peptide (VIP), substance P and CART-positive neurons.
The group that received a higher dose of FB demonstrated an increase in both the size and complexity of the complete bone neuronal network together with heightened sympathetic and sensory innervation, and displayed a decrease in neuron density and sympathetic innervation. Fumonisin B exposure led to a decrease in galanin-positive and VIP-positive bone neuronal networks in both groups exposed to FB, while in the lower-dose group, there was also a decrease in CART-positive innervation.
Prenatal FB exposure significantly influences the neuronal bone network of rats, which is essential for maintaining bone homeostasis. These findings emphasise the necessity for further research to understand the lasting effects and underlying mechanisms of alterations induced by FB.
本研究探讨了产前暴露于伏马毒素B（FB）对新生Wistar大鼠骨神经支配的影响。
从妊娠第7天至分娩，将怀孕母鼠（每组n = 6）分为对照组或两个FB暴露组之一（60毫克或90毫克/千克体重）。在分娩当天，从每窝中随机选取一只雄性幼崽（每组n = 6）进行安乐死，并解剖其股骨进行分析。通过检查交感神经、副交感神经、感觉神经和可卡因及苯丙胺调节转录物（CART）阳性纤维的形态模式来量化骨神经支配。使用免疫组织化学染色分析制备的骨切片，以检测蛋白质基因产物9.5、酪氨酸羟化酶、胆碱乙酰转移酶、血管活性肠肽（VIP）、P物质和CART阳性神经元。
接受较高剂量FB的组显示完整骨神经元网络的大小和复杂性增加，同时交感神经和感觉神经支配增强，且神经元密度和交感神经支配减少。伏马毒素B暴露导致两个FB暴露组中甘丙肽阳性和VIP阳性骨神经元网络减少，而在低剂量组中，CART阳性神经支配也减少。
产前暴露于FB会显著影响大鼠的骨神经元网络，而该网络对于维持骨稳态至关重要。这些发现强调了进一步研究以了解FB诱导的改变的持久影响和潜在机制的必要性。
