Thomassen M S, Helgerud P, Norum K R
Biochem J. 1985 Jan 15;225(2):301-6. doi: 10.1042/bj2250301.
The ability of rat small intestine to chain-shorten C22:1 fatty acids was investigated. Radioactive chain-shortened products, mainly C18:1, were demonstrated in intestinal-lymph lipids after intraluminal injection of [14-14C]erucic acid. Chain-elongation to C24:1 was also observed. Adaptation to a diet containing C22:1 fatty acids (partially hydrogenated-marine-oil diet) slightly increased the percentage of chain-shortened products. Microperoxisomal beta-oxidation activity, measured as CN(-)-insensitive palmitoyl-CoA-dependent NAD+ reduction, was detected in a microperoxisome-enriched fraction from mucosal scrapings. This activity was increased 1.9-fold by a soya-bean-oil diet, and 2.7-fold by a diet containing partially hydrogenated marine oil.
研究了大鼠小肠对C22:1脂肪酸进行链缩短的能力。腔内注射[14-14C]芥酸后，在肠淋巴脂质中证实了放射性链缩短产物，主要是C18:1。还观察到链延长至C24:1。适应含C22:1脂肪酸的饮食（部分氢化鱼油饮食）会使链缩短产物的百分比略有增加。在来自黏膜刮片的富含微过氧化物酶体的组分中检测到微过氧化物酶体β-氧化活性，以CN(-)不敏感的棕榈酰辅酶A依赖性NAD+还原为指标。大豆油饮食使该活性增加1.9倍，含部分氢化鱼油的饮食使其增加2.7倍。
