Christophersen B, Krogstad T, Norseth J
Lipids. 1983 Feb;18(2):137-41. doi: 10.1007/BF02536108.
The metabolism of [14-14C]erucic acid and [U-14C]palmitic acid has been investigated in adipocytes isolated from rat epididymal fat. The rate of acylation of [14C]erucic acid in cellular lipids and oxidation to CO2 and acid-soluble activity was ca. 1/3 of the rate with [14C]palmitic acid as substrate. A maximal incorporation of fatty acids in triacylglycerol was found at a fatty acid concentration of 0.8 mM in the medium, both with [14C]erucic acid and [14C]palmitic acid as substrate. Glucose added to the medium increased the esterification and decreased the oxidation of both fatty acids. No significant chain-shortening of [14C]erucic acid to shorter monoenes was identified in the fat cells. Increasing concentrations of unlabeled palmitic acid in the incubation medium markedly inhibited the esterification of [14C]erucic acid, whereas unlabeled erucic acid had little effect on the rate of esterification of [14C]palmitic acid.
对从大鼠附睾脂肪中分离出的脂肪细胞内[14-14C]芥酸和[U-14C]棕榈酸的代谢情况进行了研究。细胞脂质中[14C]芥酸的酰化速率以及其氧化为二氧化碳和酸溶性活性产物的速率约为以[14C]棕榈酸为底物时速率的1/3。以[14C]芥酸和[14C]棕榈酸为底物时，在培养基中脂肪酸浓度为0.8 mM时，发现脂肪酸在三酰甘油中的掺入量最大。向培养基中添加葡萄糖会增加两种脂肪酸的酯化作用，并降低其氧化作用。在脂肪细胞中未发现[14C]芥酸显著缩短为较短单烯的情况。孵育培养基中未标记棕榈酸浓度的增加显著抑制了[14C]芥酸的酯化作用，而未标记芥酸对[14C]棕榈酸的酯化速率影响很小。
