Bagheri Saeede, Hajiabadi Fatemeh, Vahabzadeh Reihaneh, Ahmadi Mohammad Hossein
Department of Laboratory Sciences, School of Paramedical and Rehabilitation Sciences, Mashhad University of Medical Sciences, Mashhad, Iran.
Student Research Committee, Mashhad University of Medical Sciences, Mashhad, Iran.
Vox Sang. 2025 Apr;120(4):354-365. doi: 10.1111/vox.13794. Epub 2025 Jan 8.
Although transfusion reactions occur in less than 2% of recipients, they are currently one of the most serious concerns in blood transfusion. Damage-associated molecular patterns (DAMPs) are released from injured, stressed or dead cells, leading to inflammation and immune system activation. One of the recognized DAMPs is mitochondrial DNA (mtDNA). It is found in various blood products, including fresh frozen plasma (FFP), red blood cell units (RBCUs) and platelet concentrates (PCs), and can induce adverse reactions in recipients by stimulating the innate immune system and inflammatory cellular pathways. The aim of this study was to investigate the factors influencing the release of mtDNA in various blood products and its subsequent impact on transfusion reactions.
In this study, mtDNA, mitochondrial DNA, mtDNA DAMPs, extracellular mtDNA, blood products, blood components and transfusion reactions between 2009 and 2023 were searched in Google Scholar, PubMed and Scopus databases.
This study has demonstrated the presence of mtDNA in the extracellular milieu of various blood products, including PCs, FFP and RBCUs. Understanding the determinants of mtDNA release and its implications for transfusion safety is critical. Strategies aimed at reducing mtDNA release, such as optimizing preparation techniques and donor selection criteria, hold promise for reducing transfusion-related complications.
By addressing these factors, healthcare providers can enhance the safety and efficacy of blood transfusion practices, ultimately improving patient outcomes.
尽管输血反应在不到2%的受血者中发生，但它们目前是输血中最严重的问题之一。损伤相关分子模式（DAMPs）从受损、应激或死亡的细胞中释放出来，导致炎症和免疫系统激活。一种公认的DAMPs是线粒体DNA（mtDNA）。它存在于各种血液制品中，包括新鲜冰冻血浆（FFP）、红细胞单位（RBCUs）和血小板浓缩物（PCs），并且可以通过刺激先天免疫系统和炎症细胞途径在受血者中引发不良反应。本研究的目的是调查影响各种血液制品中mtDNA释放的因素及其对输血反应的后续影响。
在本研究中，在谷歌学术、PubMed和Scopus数据库中搜索了2009年至2023年间的mtDNA、线粒体DNA、mtDNA DAMPs、细胞外mtDNA、血液制品、血液成分和输血反应。
本研究证明了mtDNA存在于各种血液制品的细胞外环境中，包括PCs、FFP和RBCUs。了解mtDNA释放的决定因素及其对输血安全的影响至关重要。旨在减少mtDNA释放的策略，如优化制备技术和献血者选择标准，有望减少输血相关并发症。
通过解决这些因素，医疗保健提供者可以提高输血实践的安全性和有效性，最终改善患者的治疗效果。
