Tonoike H, Kametani F, Hoshi A, Shinoda T, Isobe T
Biochem Biophys Res Commun. 1985 Feb 15;126(3):1228-34. doi: 10.1016/0006-291x(85)90317-1.
The complete amino acid sequence of the variable region of a Bence Jones protein NIG-77 from an individual with myeloma-associated systemic amyloidosis has been determined. This protein represents a complete light chain consisting of 216 residues and it has a sequence characteristic of V lambda I subgroup, which is closely homologous to that of another amyloidogenic V lambda I Bence Jones protein NIG-51, differing by 20 of 111 residues (82% homology). In contrast, it differs by 29 residues (74% homology) to that of non-amyloidogenic V lambda I light chain NIG-64. This finding shows that, in accordance with our previous report(1), the V lambda I-related light chains can further be divided into two distinct subsubgroups, V lambda I-1 and V lambda I-2, and the latter property seems to be more prone in association with the amyloid process.
已确定来自一名患有骨髓瘤相关性系统性淀粉样变性患者的本-周蛋白NIG-77可变区的完整氨基酸序列。该蛋白代表一条由216个残基组成的完整轻链，具有VλI亚组的序列特征，与另一种致淀粉样变性的VλI本-周蛋白NIG-51密切同源，在111个残基中有20个不同（同源性为82%）。相比之下，它与非致淀粉样变性的VλI轻链NIG-64有29个残基不同（同源性为74%）。这一发现表明，根据我们之前的报告(1)，VλI相关轻链可进一步分为两个不同的亚亚组，VλI-1和VλI-2，后者似乎更易于与淀粉样变过程相关联。
