Regis Wanessa Fernandes Matias, Mustafa Gomes Muniz Francisco Wilker, Rocha Francisco Ruliglésio, de Araújo Sales Ernanda Maria, Guedes Peixoto Vieira Pedro Henrique Acioly, Barros Myrna Maria Arcanjo Frota, de Almeida Alves Stephanie, Brilhante Raimunda Sâmia Nogueira, Duarte Simone, Neves Beatriz Gonçalves, Rodrigues Lidiany Karla Azevedo
Department of Operative Dentistry, Postgraduate Program in Dentistry, Faculty of Pharmacy, Dentistry and Nursing, Federal University of Ceará, Fortaleza, Ceará, Brazil.
Department of Periodontology, Federal University of Pelotas, Rio Grande do Sul, Pelotas, Brazil.
Clin Oral Investig. 2025 Jan 9;29(1):52. doi: 10.1007/s00784-024-06140-0.
This cross-sectional study aimed to evaluate the occurrence of Streptococcus spp., Streptococcus mutans, its serotypes (c, e, f, and k), collagen-binding genes (cnm/cbm), and Candida albicans in medium deep (D2) and deep (D3) dentin carious lesions of permanent teeth.
Carious dentin was collected from D2 (n = 23) and D3 (n = 24) lesions in posterior teeth from 31 individuals. DNA was extracted and analyzed using polymerase chain reaction (PCR).
Streptococcus spp. exhibited a high prevalence in both D2 and D3 lesions, with a 100% occurrence rate in D3. Although S. mutans was prevalent in both lesion types, serotype distribution highlighted differences in complexity. Serotype e was the most frequently detected in D2 samples. Serotype c was absent in D2 but present in 19.0% of D3; serotype k was exclusively found in multi-serotype D3 alongside f, c, and e. The cbm gene and undetermined serotypes were detected only in D3. Candida albicans was not found in any samples, regardless of lesion depth.
Dentinal lesions exhibited a complex array of S. mutans serotypes, with a notable prevalence of the genus Streptococcus and an overall S. mutans prevalence of approximately 35%. Candida albicans was absent. In addition, a high diversity of serotypes was observed, including multiple and indeterminate serotypes, along with the presence of the collagen-binding cbm gene.
The identification of diverse Streptococcus mutans serotypes and the cbm gene in carious dentin from permanent teeth offers insights into microbial factors potentially linked to dentinal caries lesions. Additionally, the absence of Candida albicans suggests a limited role in this context.
本横断面研究旨在评估变形链球菌及其血清型（c、e、f和k）、胶原结合基因（cnm/cbm）以及白色念珠菌在恒牙中深龋（D2）和深龋（D3）牙本质龋损中的发生情况。
从31名个体的后牙D2（n = 23）和D3（n = 24）龋损中收集龋坏牙本质。提取DNA并使用聚合酶链反应（PCR）进行分析。
链球菌属在D2和D3龋损中均具有较高的患病率，在D3中的发生率为100%。尽管变形链球菌在两种龋损类型中均普遍存在，但血清型分布突出了复杂性的差异。血清型e在D2样本中最常被检测到。血清型c在D2中不存在，但在19.0%的D3中存在；血清型k仅在多血清型D3中与f、c和e同时被发现。cbm基因和未确定的血清型仅在D3中被检测到。无论龋损深度如何，在任何样本中均未发现白色念珠菌。
牙本质龋损表现出一系列复杂的变形链球菌血清型，链球菌属患病率显著，变形链球菌总体患病率约为35%。未发现白色念珠菌。此外，观察到血清型具有高度多样性，包括多种和不确定的血清型，同时存在胶原结合cbm基因。
在恒牙龋坏牙本质中鉴定出多种变形链球菌血清型和cbm基因，有助于深入了解可能与牙本质龋损相关的微生物因素。此外，白色念珠菌的缺失表明其在这种情况下作用有限。
