Polymerase chain reaction-based identification of various serotypes of in adults with and without dental caries.

BACKGROUND

Dental caries is a multistep process which initiates the development of plaque' defined as a structured biofilm containing microbial communities. Teeth provide unique surfaces for bacterial colonization. Serotypes of implicate the development of dental caries.

AIM

The aim of the study was to determine the prevalence and association of serotypes of S. mutans in groups with and without dental caries.

MATERIALS AND METHODS

One hundred and fifty adults aged between 18 and 35 years were included in the study. Supragingival plaque samples were collected, followed by deoxyribonucleic acid extraction. Polymerase chain reaction was performed to identify and its serotypes. Proportions of and its serotypes were correlated with caries-active (CA) and caries-free (CF) groups.

RESULTS

CA group showed 66.7% positivity for and CF group showed only 42.7% of positivity. Serotype C showed a higher proportion followed by E' F, and K in the CA group, whereas in the CF group, higher proportion was observed with K followed by C' E, and F. 70.8% cases showed single serotype in the CA group and 83.3% in CF group. Multiple serotypes were seen in 29.2% in the CA group and 16.7% in the CF group.

CONCLUSIONS

The study clearly established variation in proportions of and its serotypes between CA and CF groups. Positive correlation was observed in the CA group for and its serotypes.