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来自法国全国土壤监测调查的微生物参数协调数据集。

Harmonized Datasets of microbiological parameters from a French national-scale soil monitoring survey.

作者信息

Cottin Aurélien, Dequiedt Samuel, Djemiel Christophe, Prévost-Bouré Nicolas Chemidlin, Tripied Julie, Lelièvre Mélanie, Terreau Lucie, Régnier Tiffanie, Karimi Battle, Jolivet Claudy, Bispo Antonio, Saby Nicolas, Maron Pierre-Alain, Ranjard Lionel, Terrat Sébastien

机构信息

Agroécologie, INRAE, Institut Agro, Univ. Bourgogne, Univ. Bourgogne Franche-Comté, F-21000, Dijon, France.

INRAE, InfoSol, F-45075, Orléans, France.

出版信息

Sci Data. 2025 Jan 8;12(1):34. doi: 10.1038/s41597-024-04318-5.

DOI:10.1038/s41597-024-04318-5
PMID:39779689
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11711283/
Abstract

Microbiological datasets and associated environmental parameters from the French soil quality monitoring network (RMQS) offer an opportunity for long-term and large-scale soil quality monitoring. Soils supply important ecosystem services e.g. carbon dynamics/storage or mineral element recycling, supported by the soil microbial diversity (bacteria, archaea and fungi). Based on the 2,240 sites of the 2000-2015 RMQS, molecular tools were applied to characterize soil microbiota. Soil DNA analysis yielded molecular microbial biomass for 2,168 sites, bacterial and fungal qPCR for 2,073 sites, and high-throughput amplicon sequencing of targeted 16S rDNA bacterial and archaeal genes for 1,842 sites. All these datasets were partially or completely unavailable, so raw results files from RMQS microbiological studies were harmonized and published in a Dataverse repository to facilitate their reusability. Altogether, these datasets allow for in-depth studies of soil microbial ecology and biogeography, and will be updated with fungal datasets and the second currently ongoing monitoring campaign (2016-2027).

摘要

来自法国土壤质量监测网络(RMQS)的微生物数据集及相关环境参数为长期、大规模的土壤质量监测提供了契机。土壤提供重要的生态系统服务,例如碳动态/储存或矿质元素循环,这些均由土壤微生物多样性(细菌、古菌和真菌)支撑。基于2000 - 2015年RMQS的2240个站点,运用分子工具对土壤微生物群进行表征。土壤DNA分析得出了2168个站点的分子微生物生物量、2073个站点的细菌和真菌定量聚合酶链反应(qPCR)结果,以及1842个站点的靶向16S rDNA细菌和古菌基因的高通量扩增子测序结果。所有这些数据集部分或完全不可获取,因此RMQS微生物研究的原始结果文件经过整理后在一个数据共享库中发布,以促进其可重复使用性。总体而言,这些数据集有助于深入研究土壤微生物生态学和生物地理学,并将随着真菌数据集以及当前正在进行的第二次监测活动(2016 - 2027年)进行更新。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c348/11711283/55ed379ede52/41597_2024_4318_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c348/11711283/0d2cd648b112/41597_2024_4318_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c348/11711283/c68f7fb14b16/41597_2024_4318_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c348/11711283/43a2f8df7453/41597_2024_4318_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c348/11711283/55ed379ede52/41597_2024_4318_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c348/11711283/0d2cd648b112/41597_2024_4318_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c348/11711283/c68f7fb14b16/41597_2024_4318_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c348/11711283/43a2f8df7453/41597_2024_4318_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c348/11711283/55ed379ede52/41597_2024_4318_Fig4_HTML.jpg

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本文引用的文献

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2
Global hotspots for soil nature conservation.全球土壤自然保护热点地区。
Nature. 2022 Oct;610(7933):693-698. doi: 10.1038/s41586-022-05292-x. Epub 2022 Oct 12.
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Analysis of complex trophic networks reveals the signature of land-use intensification on soil communities in agroecosystems.分析复杂的营养网络揭示了土地利用集约化对农业生态系统土壤群落的特征。
Sci Rep. 2021 Sep 14;11(1):18260. doi: 10.1038/s41598-021-97300-9.
4
BIOCOM-PIPE: a new user-friendly metabarcoding pipeline for the characterization of microbial diversity from 16S, 18S and 23S rRNA gene amplicons.BIOCOM-PIPE:一个新的用户友好型宏条形码分析工作流程,用于从 16S、18S 和 23S rRNA 基因扩增子中对微生物多样性进行特征分析。
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5
Biogeography of soil bacteria and archaea across France.法国土壤细菌和古菌的生物地理学分布
Sci Adv. 2018 Jul 4;4(7):eaat1808. doi: 10.1126/sciadv.aat1808. eCollection 2018 Jul.
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Mapping and predictive variations of soil bacterial richness across France.法国土壤细菌丰富度的图谱绘制与预测变化
PLoS One. 2017 Oct 23;12(10):e0186766. doi: 10.1371/journal.pone.0186766. eCollection 2017.
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