Lv Chao, Abdullah Mohd, Su Chun-Li, Chen Weiye, Zhou Nan, Cheng Zile, Chen Yiwen, Li Min, Simpson Kenneth W, Elsaadi Ahmed, Zhu Yongzhang, Lipkin Steven M, Chang Yung-Fu
School of Global Health, Chinese Center for Tropical Diseases Research, Shanghai Jiao Tong University School of Medicine, Shanghai, China.
National Institute of Parasitic Diseases, Chinese Center for Disease Control and Prevention (Chinese Center for Tropical Diseases Research), National Health Commission Key Laboratory of Parasite and Vector Biology, WHO Collaborating Centre for Tropical Diseases, National Center for International Research on Tropical Diseases, Shanghai, 200025, China.
BMC Genomics. 2025 Jan 8;26(1):19. doi: 10.1186/s12864-024-11198-x.
The E. coli strains harboring the polyketide synthase (pks) island encode the genotoxin colibactin, a secondary metabolite reported to have severe implications for human health and for the progression of colorectal cancer. The present study involves whole-genome-wide comparison and phylogenetic analysis of pks harboring E. coli isolates to gain insight into the distribution and evolution of these organisms. Fifteen E. coli strains isolated from patients with ulcerative colitis (UC) were sequenced, 13 of which harbored pks islands. In addition, 2,654 genomes from the public database were also screened for pks harboring E. coli genomes, 158 of which were pks-positive (pks) isolates. Whole-genome-wide comparison and phylogenetic analysis revealed that 171 (158 + 13) pks isolates belonged to phylogroup B2, and most of the isolates belong to sequence types ST73 and ST95. One isolate from a UC patient was of the sequence type ST8303. The maximum likelihood tree based on the core genome of pks isolates revealed horizontal gene transfer across sequence types and serotypes. Virulome and resistome analyses revealed the0020preponderance of virulence genes and a reduced number of antimicrobial genes in pks isolates. This study significantly contributes to understanding the evolution of pks islands in E. coli.
携带聚酮合酶（pks）岛的大肠杆菌菌株编码基因毒素大肠杆菌素，这是一种据报道对人类健康和结直肠癌进展有严重影响的次生代谢产物。本研究涉及对携带pks的大肠杆菌分离株进行全基因组比较和系统发育分析，以深入了解这些生物体的分布和进化。对从溃疡性结肠炎（UC）患者中分离出的15株大肠杆菌菌株进行了测序，其中13株携带pks岛。此外，还从公共数据库中筛选了2654个基因组以寻找携带pks的大肠杆菌基因组，其中158个是pks阳性（pks）分离株。全基因组比较和系统发育分析表明，171株（158 + 13）pks分离株属于B2系统发育群，且大多数分离株属于序列类型ST73和ST95。一名UC患者的分离株属于序列类型ST8303。基于pks分离株核心基因组的最大似然树显示序列类型和血清型之间存在水平基因转移。病毒组和耐药组分析揭示了pks分离株中毒力基因占优势且抗菌基因数量减少。本研究对理解大肠杆菌中pks岛的进化有重大贡献。
