Poursakhi SaeidReza, Asadi-Gharneh Hossein Ali, Nasr-Esfahani Mehdi, Abbasi Zahra, Hassanzadeh Khankahdani Hamed
Department of Horticulture, College of Agriculture, Esfahan Branch (Khorasgan), Islamic Azad University, Isfahan, Iran.
Plant Protection Research Department, Esfahan Agriculture and Natural Resource Research and Education Center, Esfahan, AREEO, Iran.
Physiol Plant. 2025 Jan-Feb;177(1):e70042. doi: 10.1111/ppl.70042.
In this research, we analyzed Random Amplified Polymorphic DNA (RAPD), Inter Simple Sequence Repeats (ISSR) and Sequence-related amplified polymorphism (SRAP) markers to evaluate the genetic diversity of eighteen different onion genotypes with various resistant levels to FOC. The results showed that the polymorphism means between RAPD primers was 61.11 to 81.81%; ISSR primers, 62.50 to 81.81%; and SRAP primers, 56.25 to 76.25%. Overall, by assessing MI, PIC, I and H indices, indicating the best thrive in evaluating the genetic diversity of the related onion populations. There is a significant correlation between the generated dendrograms based on similarity matrices. The classification pattern in dendrograms shows a corresponding correlation with the FOC disease severity bunches. So in all three markers studied, 'Saba' and 'Saba - HS', the most resistant ones to FOC disease, were grouped in a branch, and the 'Sahar - HS' and 'Golden Eye', the most susceptible ones were also grouped in another branch separately. This finding indicates that predominant primers act as markers linked to resistance gene(s) against FOC, which can be used to select onions resistant to FOC disease in any breeding scheme.
在本研究中，我们分析了随机扩增多态性DNA（RAPD）、简单重复序列区间（ISSR）和相关序列扩增多态性（SRAP）标记，以评估18种对尖孢镰刀菌（FOC）具有不同抗性水平的不同洋葱基因型的遗传多样性。结果表明，RAPD引物之间的多态性均值为61.11%至81.81%；ISSR引物为62.50%至81.81%；SRAP引物为56.25%至76.25%。总体而言，通过评估MI、PIC、I和H指数，表明其在评估相关洋葱种群的遗传多样性方面表现最佳。基于相似性矩阵生成的树状图之间存在显著相关性。树状图中的分类模式与FOC病害严重程度组呈现出相应的相关性。因此，在所有研究的三种标记中，对FOC病害抗性最强的‘Saba’和‘Saba - HS’被归为一个分支，而最易感的‘Sahar - HS’和‘Golden Eye’也分别被归为另一个分支。这一发现表明，主要引物可作为与抗FOC基因相关的标记，可用于在任何育种方案中筛选抗FOC病害的洋葱。
