Huang Mei, Li Xin, Chen Zhenxia, Wang Dan, Wang Kai, Hu Xuan, Chen Xiaolu, Guan Lingliang, Zhang Hongrui, Pang Yuxin, Yu Fulai
Tropical Crops Genetic Resources Institute, Chinese Academy of Tropical Agricultural Sciences, Haikou, China.
Key Laboratory of Biology and Cultivation of Herb Medicine (Haikou), Ministry of Agriculture and Rural Affairs, Haikou, China.
PLoS One. 2025 Jul 17;20(7):e0328403. doi: 10.1371/journal.pone.0328403. eCollection 2025.
Blumea balsamifera is a widely used ancient medicinal herb in tropical and subtropical Asia. Studying the genetic diversity of B. balsamifera germplasm resources and developing molecular markers related to the content of active constituents in B. balsamifera are of great significance for breeding varieties with high active ingredient content. In this study, the contents of 6 key active constituents were determined in 51 B. balsamifera accessions. EST-SSR markers were used to study their genetic diversity. At the same time, genetic structure analysis and linkage disequilibrium analysis were performed. On this basis, association analysis between EST-SSR markers and active ingredient content was performed. Quantitative analysis revealed substantial variation in six bioactive compounds, with coefficients of variation (CV) exceeding 50% across all metabolites. This result revealed its suitability for association analysis. Genetic diversity analysis revealed a total of 102 alleles amplified from 22 pairs of primers, with effective alleles accounting for 53.52%. The average polymorphism information content was 0.488, with 9 pairs of primers exhibiting high polymorphism (PIC > 0.5), 11 pairs of primers showing moderate polymorphism (0.25 < PIC < 0.5). The proposed primers demonstrated strong effectiveness and good polymorphism. The average Nei diversity index and Shannon information index were 0.542 and 1.023, respectively, indicating a high level of genetic diversity within the population. UPGMA clustering and population structure analyses classified the 51 accessions into four distinct groups correlated with geographical origins. LD analysis indicated over 50% of marker pairs showed substantial linkage (D' > 0.5), validating the suitability of this germplasm set for association mapping. Association analysis between quality traits and EST-SSR markers showed that, in both GLM and MLM models, Bbf137 and Bbf377 were found to be associated with 3,3',5,7-tetrahydroxy-4'- methoxyflavanone (TMF, P < 0.05) and Blumeatin (P < 0.01) respectively. These findings lay a robust foundation for future breeding strategies and genetic enhancement of B. balsamifera.
艾纳香是热带和亚热带亚洲广泛使用的一种古老草药。研究艾纳香种质资源的遗传多样性并开发与艾纳香活性成分含量相关的分子标记，对于培育高活性成分含量的品种具有重要意义。本研究测定了51份艾纳香种质中6种关键活性成分的含量。利用EST-SSR标记研究其遗传多样性。同时，进行了遗传结构分析和连锁不平衡分析。在此基础上，开展了EST-SSR标记与活性成分含量的关联分析。定量分析显示6种生物活性化合物存在显著差异，所有代谢物的变异系数（CV）超过50%。这一结果表明其适用于关联分析。遗传多样性分析显示，从22对引物中共扩增出102个等位基因，有效等位基因占53.52%。平均多态性信息含量为0.488，其中9对引物表现出高多态性（PIC > 0.5），11对引物表现出中等多态性（0.25 < PIC < 0.5）。所设计的引物显示出较强的有效性和良好的多态性。平均Nei多样性指数和香农信息指数分别为0.542和1.023，表明群体内遗传多样性水平较高。UPGMA聚类和群体结构分析将51份种质分为与地理起源相关的4个不同类群。连锁不平衡分析表明，超过50%的标记对表现出显著连锁（D' > 0.5），验证了该种质集适用于关联作图。品质性状与EST-SSR标记的关联分析表明，在GLM和MLM模型中，Bbf137和Bbf377分别与3,3',5,7-四羟基-4'-甲氧基黄酮（TMF，P < 0.05）和艾纳香素（P < 0.01）相关。这些发现为艾纳香未来的育种策略和遗传改良奠定了坚实基础。
