Hirose Yoshie, Fujita Chiharu, Hyoudou Tomoka, Inoue Eisuke, Inoue Hajime
Dermatology, Ginza Yoshie Clinic, Tokyo, JPN.
Regenerative Medicine, Laboratory of Cell Applied Technologies Co., Tokyo, JPN.
Cureus. 2024 Dec 9;16(12):e75405. doi: 10.7759/cureus.75405. eCollection 2024 Dec.
Recently, autologous cultured fibroblast and platelet-rich plasma (PRP) therapies have been attempted for skin rejuvenation. Unlike PRP, grafted fibroblasts not only produce connective tissue but also influence the surrounding environment through a paracrine effect. Fibroblast-derived cytokines interact with and are modulated by neighboring tissue-constituting cells. In this study, we aimed to perform autologous fibroblast therapy and examine its effectiveness for skin rejuvenation through patient and doctor evaluations.
Eighty-eight patients (5 males and 83 females) were followed up three months after grafting. All cases had a chief complaint of age-related skin atrophy. 1x10 cells of autologous cultured dermal fibroblasts were administered to each face. The patient and doctors evaluated the treatment's effects at one and three months.
One-month post-treatment, 60.3% of the patients rated the treatment as effective, while the doctors evaluated 79.5% as effective. Due to these findings, we assessed the efficacy of the eighty-eight patients at three months by the last observation carried forward (LOCF). The results showed that 75% and 92%, respectively, had effective patient and doctor assessments. The effects of fibroblast treatment were more effective after three months.
Fibroblast grafting was more effective at three months than one month and was extremely effective in improving skin texture, such as sagging, firmness, and wrinkles, without the symptoms of large depressions. There was a natural improvement more than hyaluronic acid injection.
最近，自体培养成纤维细胞和富血小板血浆（PRP）疗法已被尝试用于皮肤年轻化。与PRP不同，移植的成纤维细胞不仅能产生结缔组织，还能通过旁分泌作用影响周围环境。成纤维细胞衍生的细胞因子与相邻的组织构成细胞相互作用并受到其调节。在本研究中，我们旨在进行自体成纤维细胞治疗，并通过患者和医生评估来检验其对皮肤年轻化的有效性。
88例患者（5例男性和83例女性）在移植后3个月进行随访。所有病例的主要诉求均为与年龄相关的皮肤萎缩。将1×10个自体培养的真皮成纤维细胞注射到每侧面部。患者和医生在1个月和3个月时评估治疗效果。
治疗1个月后，60.3%的患者认为治疗有效，而医生评估的有效率为79.5%。基于这些结果，我们采用末次观察结转（LOCF）法评估了88例患者在3个月时的疗效。结果显示，患者和医生评估的有效率分别为75%和92%。成纤维细胞治疗在3个月后的效果更佳。
成纤维细胞移植在3个月时比1个月时更有效，在改善皮肤质地（如松弛、紧致度和皱纹）方面极为有效，且无大凹陷症状。其改善效果比透明质酸注射更自然。
