Ngwenya B Z, Yamamoto N
Biochim Biophys Acta. 1985 Mar 29;839(1):9-15. doi: 10.1016/0304-4165(85)90175-8.
Lysophosphatidylcholine (lyso-PC), a product of inflammation induced by infectious and other agents, is able to stimulate mouse peritoneal macrophages to ingest target cells coated with IgG but not IgM regardless of the presence of complement. In vitro treatment of mouse resident peritoneal macrophages (adherent cells) alone with lyso-PC stimulated spreading activity but did not enhance ingestion activity of macrophages. However, when mixed cultures of adherent and nonadherent (lymphocytes) cells were treated with lyso-PC, macrophage ingestion activity of IgG-coated target cells (i.e., via Fc-mediated ingestion) was markedly enhanced. Analysis of lyso-PC activation process of macrophages for ingestion activity suggests that nonadherent (lymphocytes) cells are required for the induction of the manifestation of ingestion capacity. This requirement was also met by addition of untreated nonadherent cells to treated adherent cells. Thus, the activation mechanism of macrophages by lyso-PC for ingestion requires contribution of lymphocytes to promote enhanced ingestion activity. Since lyso-PC is a metabolite of a representative membrane phospholipid, we propose that lyso-PC and other lysophospholipids are mediators for activation of macrophages regardless of the type of inflammation-causative agent.
溶血磷脂酰胆碱（lyso-PC）是由感染及其他因素诱导产生的炎症产物，它能够刺激小鼠腹腔巨噬细胞摄取包被有IgG而非IgM的靶细胞，且不受补体存在与否的影响。单独用lyso-PC体外处理小鼠驻留腹腔巨噬细胞（贴壁细胞）可刺激其铺展活性，但不会增强巨噬细胞的摄取活性。然而，当用lyso-PC处理贴壁细胞和非贴壁细胞（淋巴细胞）的混合培养物时，巨噬细胞对包被有IgG的靶细胞的摄取活性（即通过Fc介导的摄取）会显著增强。对巨噬细胞摄取活性的lyso-PC激活过程分析表明，摄取能力的表现诱导需要非贴壁细胞（淋巴细胞）。将未处理的非贴壁细胞添加到处理过的贴壁细胞中也满足了这一需求。因此，lyso-PC激活巨噬细胞摄取的机制需要淋巴细胞的参与来促进增强的摄取活性。由于lyso-PC是一种代表性膜磷脂的代谢产物，我们提出lyso-PC和其他溶血磷脂是巨噬细胞激活的介质，而与炎症致病因子的类型无关。
