Ngwenya B Z, Yamamoto N
Cancer Immunol Immunother. 1986;21(3):174-82. doi: 10.1007/BF00199358.
Microbial infection causes inflammation which stimulates macrophage functions. One of the inflammatory products, lysophosphatidylcholine (lyso-Pc), can stimulate macrophage activities. Treatment of mice with lyso-Pc enhanced spreading and ingestion activities of peritoneal macrophages. In vitro treatment of macrophages with lyso-Pc greatly enhanced spreading but not ingestion activities. However, incubation of a mixture of adherent and nonadherent cells with lyso-Pc produced a markedly enhanced ingestion activity of macrophages, implying the contribution of nonadherent cells to the stimulation of macrophages. Time course studies of the stimulation of these macrophages showed that spreading activity is stimulated immediately, even 30 min, after their contact with lyso-Pc while induction of ingestion activity requires a latent period of about 5 h. When the specificity of the macrophage receptors for ingestion was analyzed using defined immunoglobulins (i.e., IgG and IgM) with or without complement, lyso-Pc-activated macrophages efficiently ingested IgG-coated sheep erythrocytes independent of complement. However, macrophages of the same lyso-Pc-treated mice did not ingest erythrocytes coated with IgM and complement. These observations suggest that lyso-Pc-stimulated macrophages ingest the targets via Fc-receptors but not C3b receptors.
微生物感染会引发炎症，进而刺激巨噬细胞的功能。炎症产物之一溶血磷脂酰胆碱（lyso-Pc）能够刺激巨噬细胞的活性。用lyso-Pc处理小鼠可增强腹膜巨噬细胞的铺展和吞噬活性。在体外，用lyso-Pc处理巨噬细胞可显著增强其铺展活性，但对吞噬活性无增强作用。然而，将贴壁细胞和非贴壁细胞的混合物与lyso-Pc共同孵育，可使巨噬细胞的吞噬活性显著增强，这表明非贴壁细胞对巨噬细胞的刺激有贡献。对这些巨噬细胞刺激的时间进程研究表明，铺展活性在与lyso-Pc接触后立即被刺激，甚至在30分钟后仍有反应，而吞噬活性的诱导则需要约5小时的潜伏期。当使用特定的免疫球蛋白（即IgG和IgM）并结合或不结合补体来分析巨噬细胞摄取受体的特异性时，lyso-Pc激活的巨噬细胞能够有效摄取IgG包被的绵羊红细胞，且不依赖补体。然而，同样经lyso-Pc处理的小鼠的巨噬细胞不会摄取IgM和补体包被的红细胞。这些观察结果表明，lyso-Pc刺激的巨噬细胞通过Fc受体而非C3b受体摄取靶标。
