Pappu L D, Levkoff A H, Shannon B T, Loadholt C B
Biol Neonate. 1985;47(2):70-6. doi: 10.1159/000242093.
We have reported in our previous study that macrophages constitute 58% of the total number of white cells in noninfected neonatal cerebrospinal fluid (CSF). CSF macrophages are probably derived from blood monocytes. To explain their predominance, we compared the chemotactic response of monocytes from cord and adult blood to neonatal (NCSF) and non-neonatal spinal fluids (NNCSF). Zymosan-activated serum (ZAS) was used as a positive control. Significance was accepted as p less than or equal to 0.05. We observed that both random migratory (RM) activity and chemotactic responses (CM) of cord monocytes to neonatal CSF and ZAS were greater than those of monocytes obtained from adult blood, resulting in a significant increase in the chemotactic differential (CD). Chemotactic responses of neonatal monocytes were significantly greater in the presence of neonatal CSF as compared to CSF from older children as indicated by CD. The CD of neonatal monocytes to neonatal CSF was greater than that of neonatal neutrophils. These findings are consistent with the presence of specific monocyte chemotaxins in noninfected neonatal CSF.
我们在之前的研究中报告过，巨噬细胞占未感染新生儿脑脊液（CSF）中白细胞总数的58%。脑脊液巨噬细胞可能来源于血液单核细胞。为了解释它们的优势地位，我们比较了脐带血和成人血中的单核细胞对新生儿脑脊液（NCSF）和非新生儿脑脊液（NNCSF）的趋化反应。酵母聚糖激活血清（ZAS）用作阳性对照。显著性水平设定为p小于或等于0.05。我们观察到，脐带血单核细胞对新生儿脑脊液和ZAS的随机迁移（RM）活性和趋化反应（CM）均大于成人血单核细胞，导致趋化差异（CD）显著增加。如CD所示，与大龄儿童的脑脊液相比，新生儿单核细胞在新生儿脑脊液存在时的趋化反应显著更强。新生儿单核细胞对新生儿脑脊液的CD大于新生儿中性粒细胞。这些发现与未感染新生儿脑脊液中存在特异性单核细胞趋化因子一致。
