Aradi J, Ho Y K
Cancer Biochem Biophys. 1985 Feb;7(4):349-59.
In continuation of efforts to correlate the antitemplate activities of modified polynucleotides with their structure, and to understand the factors governing both their potency and stability, a group of single-stranded poly(ribo- and deoxyribo-) nucleotides, and the "hybrid" double-stranded complexes were prepared and investigated. The double-stranded hybrid poly(A,hs5U).poly(dT) section was found to be more stable to murine blood nucleases than was the single-stranded poly(A,hs5U). In a comparative study as inhibitors of the DNA polymerase alpha from rat hepatoma, the results showed that the modified polynucleotides were more potent than the unmodified ones, in general, the polydeoxyribonucleotides were better antitemplates than their ribo counterparts and the poly(A70,hs5U30).poly(dT) hybrid was more active than either of the single-stranded components. Thus it is possible to increase the nuclease resistance of the modified polyribonucleotides by forming hybrid complexes with complementary polydeoxyribonucleotides, and at the same time, to augment their antitemplate activities.
为了继续努力将修饰的多核苷酸的抗模板活性与其结构相关联，并了解影响其效力和稳定性的因素，制备并研究了一组单链聚（核糖和脱氧核糖）核苷酸以及“杂交”双链复合物。发现双链杂交聚（A，hs5U）.聚（dT）片段对鼠血核酸酶的稳定性高于单链聚（A，hs5U）。在作为大鼠肝癌DNA聚合酶α抑制剂的比较研究中，结果表明，修饰的多核苷酸比未修饰的多核苷酸更有效，一般来说，聚脱氧核糖核苷酸比其核糖对应物是更好的抗模板，并且聚（A70，hs5U30）.聚（dT）杂交体比任何一种单链成分更具活性。因此，通过与互补的聚脱氧核糖核苷酸形成杂交复合物，可以提高修饰的聚核糖核苷酸的核酸酶抗性，同时增强其抗模板活性。
