Moan J, Sommer S
Cancer Res. 1985 Apr;45(4):1608-10.
Cells of the established line NHIK 3025 were incubated with hematoporphyrin derivative and exposed to light at different concentrations of oxygen. The efficiency of photoinactivation of the sensitized cells decreased with decreasing oxygen concentration. No photoinactivation was observed when the atmosphere above the medium overlying the cells was pure N2 gas. With 1% O2 in the atmosphere, the quantum yield of photoinactivation was reduced by 50% compared to the yield in air-saturated medium. It is hardly possible to inactivate cells in anoxic regions of a tumor by means of porphyrin sensitized photochemotherapy. In spite of this, the therapy seems to be efficient in several cases. Thus, it seems that anoxic tumor cells are inactivated in secondary reactions, probably due to breakdown of the circulatory system in the tumor.
将已建立的NHIK 3025细胞系的细胞与血卟啉衍生物一起孵育，并在不同氧浓度下进行光照。致敏细胞的光灭活效率随着氧浓度的降低而降低。当覆盖细胞的培养基上方的气体为纯氮气时，未观察到光灭活现象。与空气饱和培养基中的光灭活量子产率相比，当大气中氧含量为1%时，光灭活量子产率降低了50%。通过卟啉敏化光化学疗法几乎不可能使肿瘤缺氧区域的细胞失活。尽管如此，该疗法在一些病例中似乎是有效的。因此，似乎缺氧肿瘤细胞是在继发反应中被灭活的，这可能是由于肿瘤循环系统的破坏所致。
