Dougherty T J, Gomer C J, Weishaupt K R
Cancer Res. 1976 Jul;36(7 PT 1):2330-3.
Hematoporphryrin derivative at an intracellular concentration in TA-3 mouse mammary carcinoma cells of 0.6 or 0.9 mM required input of 3.0 X 3.6 X 10(9) quanta/cell of red light (620 nm) to achieve a 90% kill. At an intracellular concentration of 1.2 mM, this light requirement drops to 1.5 X 10(9)quanta/cell. The energy for this photodynamic process is about 100 times higher than that required for ionizing radiation to achieve the same level of kill for these cells. The quantum yield for singlet oxygen formation (the cytotoxic agent in most photodynamic processes) from hematoporphyrin derivative is 0.75 +/- 0.07 in ethanol but only 0.16 +/- 0.07 within TA-3 cells, indicating possible intracellular complexing and quenching.
在TA-3小鼠乳腺癌细胞中，血卟啉衍生物细胞内浓度为0.6或0.9 mM时，需要输入3.0×3.6×10⁹个/细胞的红光（620 nm）量子才能实现90%的细胞杀伤率。当细胞内浓度为1.2 mM时，所需光量子降至1.5×10⁹个/细胞。对于这些细胞，这种光动力过程所需的能量比实现相同杀伤水平的电离辐射所需能量高约100倍。血卟啉衍生物产生单线态氧（大多数光动力过程中的细胞毒性剂）的量子产率在乙醇中为0.75±0.07，但在TA-3细胞内仅为0.16±0.07，这表明可能存在细胞内络合和猝灭现象。
