Kemp A S, Mellis C M, Barnett D, Sharota E, Simpson J
Clin Allergy. 1985 Jan;15(1):73-8. doi: 10.1111/j.1365-2222.1985.tb02258.x.
One-hundred-and-four children were skin-tested with four peanut-allergen preparations, a commercial extract, extracts of raw and roast peanuts prepared by NH4HCO3 extraction, and a wheatgerm lectin-reactive glycoprotein obtained by affinity chromatography. The presence of symptoms after ingestion of peanut or peanut products was also recorded. The roast allergen extract provided the greatest specificity with eight symptomatic children having a positive skin test and only one positive skin-test reaction in an asymptomatic child in the group of 104 children tested. Despite differences in the incidence of skin-test reactions there was a strong correlation between raw, roast and commercial RAST suggesting common allergens were being identified by circulating IgE. Clinical sensitivity was observed particularly in younger children with 75% of the children being under 4 years of age. A positive roast skin test or a RAST test adds confirmation to the clinical history of allergic reactions to peanuts.
对104名儿童用四种花生过敏原制剂进行皮肤试验，一种是商业提取物，一种是用碳酸氢铵提取法制备的生花生和烤花生提取物，还有一种是通过亲和层析获得的麦胚凝集素反应性糖蛋白。同时记录摄入花生或花生制品后出现症状的情况。在接受测试的104名儿童中，烤过敏原提取物特异性最高，有8名出现症状的儿童皮肤试验呈阳性，而在无症状儿童组中只有1名皮肤试验呈阳性反应。尽管皮肤试验反应的发生率存在差异，但生花生、烤花生和商业性放射性过敏原吸附试验（RAST）之间存在很强的相关性，这表明循环IgE识别出了共同的过敏原。临床敏感性尤其在幼儿中观察到，75%的儿童年龄在4岁以下。烤花生皮肤试验或RAST试验呈阳性可为花生过敏反应的临床病史提供佐证。
