Barnett D, Baldo B A, Howden M E
J Allergy Clin Immunol. 1983 Jul;72(1):61-8. doi: 10.1016/0091-6749(83)90053-2.
Crude peanut protein fractions from raw and roasted peanuts were examined in the RAST with 10 sera from patients showing clinical peanut sensitivity. The radioactive uptake results, which were generally high, did not reveal any distinguishable pattern. Two commercially available peanut proteins, peanut lectin and phospholipase D, gave poor RAST responses. Three purified peanut proteins, alpha-arachin, conarachin I, and concanavalin A-reactive glycoprotein, all gave significant RAST results that were generally lower than those obtained with the crude extracts. The extent of RAST inhibition obtained with these materials was inversely related to their abundance in the total peanut protein. Crossed immunoelectrophoresis with extracts from raw and roasted peanut indicated the presence of 22 and 10 anodically migrating antigens, respectively. Sixteen IgE binding antigens were revealed for raw peanut and seven for roasted peanut after incubation with a mixed serum from the 10 patients in crossed radioimmunoelectrophoresis (CRIE) using 125I-labeled anti-IgE. CRIE plates treated with individual serum samples showed that all the patients had specific IgE for the major antigen peak, which has been tentatively identified as alpha-arachin. This major storage protein of peanut, which is known to be particularly heat resistant; may be of greater clinical significance than its apparently low RAST activity would seem to indicate.
用来自表现出临床花生过敏反应患者的10份血清，通过放射变应原吸附试验（RAST）检测了生花生和烤花生的粗花生蛋白组分。放射性摄取结果通常较高，但未显示出任何可区分的模式。两种市售花生蛋白，花生凝集素和磷脂酶D，在RAST中的反应较差。三种纯化的花生蛋白，α-花生球蛋白、伴花生球蛋白I和伴刀豆球蛋白A反应性糖蛋白，在RAST中均给出了显著结果，总体上低于粗提物所获得的结果。用这些物质获得的RAST抑制程度与它们在总花生蛋白中的丰度呈负相关。用生花生和烤花生提取物进行的交叉免疫电泳表明，分别存在22种和10种向阳极迁移的抗原。在用125I标记的抗IgE进行交叉放射免疫电泳（CRIE）中，与10名患者的混合血清孵育后，生花生显示出16种IgE结合抗原，烤花生显示出7种。用个体血清样本处理的CRIE板显示，所有患者对主要抗原峰都有特异性IgE，该主要抗原峰已初步鉴定为α-花生球蛋白。花生的这种主要储存蛋白，已知特别耐热，可能比其明显较低的RAST活性所显示的具有更大的临床意义。
