Bernard L, Delosière M, Hurtaud C, Imbert A, Viala D, Cebo C, Bonnet M
Université Clermont Auvergne, INRAE, Vetagro Sup, UMRH, 63122 Saint-Genès-Champanelle, France.
PEGASE, INRAE, Institut Agro, 35590 Saint-Gilles, France.
Food Chem. 2025 Apr 15;471:142628. doi: 10.1016/j.foodchem.2024.142628. Epub 2024 Dec 31.
A study of the lipidome and proteome was performed on milk fat globule membranes (MFGM) originating from milk samples from high (HL) and low (LL) lipolysis groups of cows. Combined univariate and multivariate statistical analyses proposed a set of variables highly associated to contrasted samples with regard to milk lipolysis. Milk from HL group were related to 4 phosphatidylinositols, 8 phosphatidylcholines, 1 sphingomyelin and 27 proteins, among them the phosphatidylcholine/phosphatidylethanolamine ratio and ORM1 may contribute to the membrane remodeling of the MFGM. The abundance of CP, CHI3L1, NECTIN2, A2M were strongly positively correlated with HL. Conversely, 3 phosphatidylinositols, 1 phosphatidylcholine and 2 phosphatidylethanolamines were assigned to the LL group. The HL group in cows is associated with a specific MFGM phospholipids and proteins profile, suggesting an impact on membrane fluidity and lipid rafts composition intervening in LPL anchoring and activation, as well as on pro-inflammatory lipids and proteins.
对来自高（HL）脂解组和低（LL）脂解组奶牛的乳样中的乳脂肪球膜（MFGM）进行了脂质组和蛋白质组研究。单变量和多变量统计分析相结合，提出了一组与乳脂解对比样本高度相关的变量。HL组的牛奶与4种磷脂酰肌醇、8种磷脂酰胆碱、1种鞘磷脂和27种蛋白质有关，其中磷脂酰胆碱/磷脂酰乙醇胺比值和ORM1可能有助于MFGM的膜重塑。CP、CHI3L1、NECTIN2、A2M的丰度与HL呈强正相关。相反，3种磷脂酰肌醇、1种磷脂酰胆碱和2种磷脂酰乙醇胺被归为LL组。奶牛中的HL组与特定的MFGM磷脂和蛋白质谱相关，这表明对膜流动性和脂质筏组成有影响，进而影响脂蛋白脂肪酶（LPL）的锚定和激活，以及促炎脂质和蛋白质。
