Malter J S, Manotti S E, Knee G R, Goodman D B, Strauss J F
Clin Chem. 1985 Apr;31(4):642-4.
We evaluated a new assay (TSH3 MAIAclone) for thyrotropin (TSH) with improved sensitivity, testing a series of hospital inpatients with increased free thyroxin indices (thyroxin concentration X triiodothyronine uptake on resin). This assay involves use of three monoclonal antibodies and an antibody-magnetic particle conjugate that rapidly and completely separates bound and free tracer in a magnetic field. The assay turnaround time is 3 h. By the TSH3-MAIAclone assay, 65% of these patients with an increased free thyroxin index were identified on the basis of a TSH value less than or equal to 0.50 milli-int. unit/L. In contrast, another commercially available assay for TSH detected suppressed TSH concentrations in less than 5% of these patients. We conclude that the TSH3 MAIAclone assay markedly improves our ability to discriminate hyperthyroidism from euthyroidism.
我们评估了一种新的促甲状腺激素（TSH）检测方法（TSH3 MAIAclone），其灵敏度有所提高，并对一系列游离甲状腺素指数升高（甲状腺素浓度×树脂上三碘甲状腺原氨酸摄取率）的住院患者进行了检测。该检测方法使用三种单克隆抗体和一种抗体 - 磁性颗粒结合物，可在磁场中快速、完全地分离结合型和游离型示踪剂。检测周转时间为3小时。通过TSH3 - MAIAclone检测方法，这些游离甲状腺素指数升高的患者中，65%是根据TSH值小于或等于0.50毫国际单位/升来确定的。相比之下，另一种市售的TSH检测方法在这些患者中检测到TSH浓度被抑制的比例不到5%。我们得出结论，TSH3 MAIAclone检测方法显著提高了我们区分甲状腺功能亢进与正常甲状腺功能的能力。
