Triwardhani Ari, Alida Alida, Winoto Ervina Restiwulan, Pramusita Adya, Putranti Nurul Aisyah Rizki, Ariadi Kristian Satrio, Pribadi Okso Brillian, Anwar Amelia Aisyiah, Purnamasari Alqomariyah Eka, Mappananrang Rifqah Ananda, Situmorang Putri Cahaya, Riawan Wibi, Noor Tengku Natasha Eleena Binti Tengku Ahmad, Nugraha Albertus Putera, Nugraha Alexander Patera
Department of Orthodontic, Faculty of Dental Medicine, Universitas Airlangga, Surabaya, Indonesia.
Faculty of Dental Medicine, Universitas Airlangga, Surabaya, Indonesia.
Eur J Dent. 2025 May;19(2):523-530. doi: 10.1055/s-0044-1791937. Epub 2025 Jan 9.
The mechanical stimulation known as orthodontic mechanical force (OMF) causes biological reactions in orthodontic tooth movement (OTM). Heat shock protein-70 (HSP-70) needs pro-inflammatory cytokines to trigger bone resorption in OTM; nevertheless, heat shock protein-10 (HSP-10), a "Alarmin" cytokine, should control these pro-inflammatory cytokines to get the best alveolar bone remodeling (ABR). L. nanosuspension extract (MONE) has anti-inflammatory, antioxidant, and ABR-stimulating properties. The aim of the study was to examine HSP-10 and HSP-70 expressions under OMF following MONE application in Wistar rats ().
A total of 36 Wistar rats () were split up into eight groups: one for treatment (OMF + MONE) and one for control (OMF + MONE administration for days 1, 7, 14, and 21). By employing nickel-titanium coil springs and using 10 g of light force per millimeter to implant the orthodontic device, the OMF was completed. According to the day of observation, all of the samples were sacrificed. To perform an immunohistochemistry investigation, the premaxilla of the sample was isolated. Tukey's Honest Significant Different (HSD) test ( < 0.05) was performed after an Analysis of Variance (ANOVA) analysis of the data.
In both the OMF and MONE groups, HSP-70 peaked on day 14 and began to fall on day 21. HSP-10 peaked on day 21, but along with MONE, it also began to progressively decline on days 14 and 21, with significant differences ( < 0.05).
According to immunohistochemistry evidence, postadministration of MONE markedly elevated HSP-10 but lowered HSP-70 expression in the alveolar bone of Wistar rats under OMF.
正畸机械力（OMF）这种机械刺激会在正畸牙齿移动（OTM）过程中引发生物学反应。热休克蛋白70（HSP - 70）需要促炎细胞因子来触发OTM中的骨吸收；然而，热休克蛋白10（HSP - 10）作为一种“警报素”细胞因子，应该控制这些促炎细胞因子以实现最佳的牙槽骨重塑（ABR）。纳米乳剂提取物（MONE）具有抗炎、抗氧化和刺激ABR的特性。本研究的目的是在Wistar大鼠中应用MONE后，检测OMF作用下HSP - 10和HSP - 70的表达情况。
总共36只Wistar大鼠被分为八组：一组用于治疗（OMF + MONE），一组用于对照（第1、7、14和21天给予OMF + MONE）。通过使用镍钛螺旋弹簧并以每毫米10克的轻力植入正畸装置来完成OMF。根据观察日，处死所有样本。分离样本的上颌前部以进行免疫组织化学研究。对数据进行方差分析（ANOVA）后，进行Tukey's 诚实显著差异（HSD）检验（P < 0.05）。
在OMF组和MONE组中，HSP - 70在第14天达到峰值，并在第21天开始下降。HSP - 10在第21天达到峰值，但与MONE一起，它在第14天和第21天也开始逐渐下降，差异有统计学意义（P < 0.05）。
根据免疫组织化学证据，在Wistar大鼠的牙槽骨中，OMF作用下MONE给药后显著提高了HSP - 10的表达，但降低了HSP - 70的表达。
