Nugraha Alexander Patera, Narmada Ida Bagus, Winoto Ervina Restiwulan, Ardani I Gusti Aju Wahju, Triwardhani Ari, Alida Alida, Pramusita Adya, Nur Reyhan Mahendra, Indrastie Nuraini, Nam Hui Yin, Ihsan Igo Syaiful, Riawan Wibi, Rantam Fedik Abdul, Nugraha Albertus Putera, Noor Tengku Natasha Eleena Binti Tengku Ahmad
Department of Orthodontics, Faculty of Dental Medicine, Universitas Airlangga, Surabaya, Indonesia.
Nanotechnology and Catalysis Research Centre (NANOCAT), Universiti Malaya, Kuala Lumpur, Malaysia.
Eur J Dent. 2024 May;18(2):501-509. doi: 10.1055/s-0043-1772699. Epub 2023 Nov 23.
The aim of this article was to investigate Osterix, ALP, and osteopontin expression in the compression and tension sides of alveolar bone after the application of normoxic/hypoxic-preconditioned GMSCs in rabbits () induced with OMF.
Forty-eight healthy, young male rabbits were divided into four groups: [-] OMF; [+] OMF; OMF with GMSCs normoxic-preconditioned; and OMF and GMSCs hypoxic-preconditioned. The central incisor and left mandibular molar in the experimental animals were moved, the mandibular first molar was moved mesially using nickel titanium (NiTi) and stainless steel ligature wire connected to a 50 g/mm light force closed coil spring. Allogeneic application of normoxic or hypoxic-preconditioned GMSCs was used in as many as 10 cells in a 20 µL phosphate buffered saline single dose and injected into experimental animals' gingiva after 1 day of OTM. On days 7, 14, and 28, all experimental animals were euthanized. Osterix, ALP, and osteopontin expressions were examined by immunohistochemistry.
Osterix, ALP, and osteopontin expressions were significantly different after allogeneic application of hypoxic-preconditioned GMSCs than normoxic-preconditioned GMSCs in the tension and compression of the alveolar bone side during OMF ( < 0.05).
Osterix, ALP, and osteopontin expressions were significantly more enhanced post-transplantation of GMSCs with hypoxic-preconditioning than after transplantation of normoxic-preconditioned GMSCs in rabbits () induced with OMF.
本文旨在研究在正畸性骨改建（OMF）诱导的兔模型中，应用常氧/低氧预处理的牙龈间充质干细胞（GMSCs）后，牙槽骨受压侧和受拉侧中osterix、碱性磷酸酶（ALP）和骨桥蛋白的表达情况。
将48只健康的年轻雄性兔分为四组：[-]OMF组；[+]OMF组；常氧预处理GMSCs的OMF组；低氧预处理GMSCs的OMF组。对实验动物的中切牙和左下颌磨牙进行移动，使用镍钛（NiTi）和不锈钢结扎丝连接50 g/mm的轻力闭合螺旋弹簧将下颌第一磨牙近中移动。将常氧或低氧预处理的GMSCs以单剂量20 μL磷酸盐缓冲盐水中多达10个细胞的异体应用，在正畸牙齿移动（OTM）1天后注射到实验动物的牙龈中。在第7、14和28天，对所有实验动物实施安乐死。通过免疫组织化学检测osterix、ALP和骨桥蛋白的表达。
在正畸性骨改建过程中，异体应用低氧预处理的GMSCs后，牙槽骨受拉侧和受压侧中osterix、ALP和骨桥蛋白的表达与常氧预处理的GMSCs相比有显著差异（P<0.05）。
在正畸性骨改建诱导的兔模型中，低氧预处理的GMSCs移植后，osterix、ALP和骨桥蛋白的表达比常氧预处理的GMSCs移植后显著增强。
