Liang Bixia, Huang Zhixi, Cheng Lingyan, Quan Jin, Mou Zongxia, Li Nan, Cui Xin
Key Laboratory of Biomaterials of Guangdong Higher Education Institutes, Department of Biomedical Engineering, Jinan University, Guangzhou, 510632, China.
Key Laboratory of Biomaterials of Guangdong Higher Education Institutes, Department of Biomedical Engineering, Jinan University, Guangzhou, 510632, China.
Anal Chim Acta. 2025 Jan 22;1336:343504. doi: 10.1016/j.aca.2024.343504. Epub 2024 Nov 30.
The multifunctional cytokine interleukin-6 (IL-6) plays a pivotal role in chronic and acute inflammatory responses, underscoring the importance of accurately determining IL-6 levels for early diagnosis, prevention, and treatment of inflammation.
This study developed a versatile and innovative single-particle surface-enhanced Raman spectroscopy (SERS) sensing platform for the precise and sensitive quantification of IL-6 in complex samples using a novel one-pot synthesized, silver ions-doped three-dimensional porous gold microparticles (PGMs) with abundant hot spots for robust SERS enhancement. By rationally designing rich cytosine-Ag-cytosine base pairs between IL-6 aptamers and complementary chains on the PGMs, we harnessed the SERS-enhancing effect to achieve highly sensitive and specific IL-6 quantification within a wide range of 10 to 10 mg/mL and a limit of detection (LOD) of 0.093 pg/mL. Moreover, the accurate detection of IL-6 in spiked serum samples exhibited a remarkable recovery rate ranging from 97.2 % to 107.6 %.
This aptamer sensor provides an alternative tool for cost-effective, sensitive and broad-range IL-6 detection, which would contribute to the early detection of inflammation and pave the way for timely interventions and improved patient outcomes.
多功能细胞因子白细胞介素-6（IL-6）在慢性和急性炎症反应中起关键作用，这突出了准确测定IL-6水平对炎症早期诊断、预防和治疗的重要性。
本研究开发了一种通用且创新的单颗粒表面增强拉曼光谱（SERS）传感平台，用于在复杂样品中精确灵敏地定量检测IL-6。该平台使用新型一锅合成的银离子掺杂三维多孔金微粒（PGM），其具有丰富的热点以实现强大的SERS增强。通过合理设计IL-6适配体与PGM上互补链之间丰富的胞嘧啶-银-胞嘧啶碱基对，我们利用SERS增强效应在10至10 mg/mL的宽范围内实现了高灵敏且特异的IL-6定量检测，检测限（LOD）为0.093 pg/mL。此外，对加标血清样品中IL-6的准确检测显示出97.2%至107.6%的显著回收率。
这种适配体传感器为经济高效、灵敏且宽范围的IL-6检测提供了一种替代工具，这将有助于炎症的早期检测，并为及时干预和改善患者预后铺平道路。
