Černoša Anja, Gostinčar Cene, Holcar Marija, Kostanjšek Rok, Lenassi Metka, Gunde-Cimerman Nina
Department of Biology, Biotechnical Faculty, University of Ljubljana, Ljubljana, Slovenia.
InnoRenew CoE, Livade 6a, Izola, 6310, Slovenia.
Sci Rep. 2025 Jan 9;15(1):1413. doi: 10.1038/s41598-024-84189-3.
Aureobasidium melanogenum is a black yeast-like fungus that occurs frequently both in nature and in domestic environments. It is becoming increasingly important as an opportunistic pathogen. Nevertheless, its effect on human cells has not yet been studied. In this study, we investigated the effect of A. melanogenum cells and extracellular vesicles (EVs) on human cell lines A549 (human lung cells), HDFa (human dermal fibroblasts), and SH-SY5Y (human neuroblastoma cells). Scanning electron microscopy (SEM) showed no direct interaction between A. melanogenum cells and human cell lines, but there were some changes in HDFa cells. As a possible cause for this change, we tested the cytotoxic effect of EVs from A. melanogenum on the same cell lines. We isolated EVs from the fungus and prepared three different pools: a non-melanin pool (containing mainly EVs), a melanin pool (containing mainly melanin nanoparticles), and a total pool (containing both EVs and melanin nanoparticles). All three pools were characterized and then added to human cell lines to test their cytotoxicity. Unlike in some other fungal opportunistic pathogens, no effects of fungal EVs on human cell viability were observed. Therefore, the opportunistic potential of A. melanogenum remains only partially understood.
黑产黄青霉是一种黑色酵母样真菌，在自然环境和家庭环境中均频繁出现。作为一种机会致病菌，它正变得越来越重要。然而，其对人类细胞的影响尚未得到研究。在本研究中，我们调查了黑产黄青霉细胞和细胞外囊泡（EVs）对人细胞系A549（人肺细胞）、HDFa（人皮肤成纤维细胞）和SH-SY5Y（人神经母细胞瘤细胞）的影响。扫描电子显微镜（SEM）显示黑产黄青霉细胞与人细胞系之间没有直接相互作用，但HDFa细胞有一些变化。作为这种变化的一个可能原因，我们测试了来自黑产黄青霉的EVs对相同细胞系的细胞毒性作用。我们从真菌中分离出EVs并制备了三个不同的组分：一个非黑色素组分（主要含有EVs）、一个黑色素组分（主要含有黑色素纳米颗粒）和一个总组分（含有EVs和黑色素纳米颗粒）。对所有三个组分进行了表征，然后添加到人细胞系中以测试它们的细胞毒性。与其他一些真菌机会致病菌不同，未观察到真菌EVs对人细胞活力有影响。因此，黑产黄青霉的机会致病潜力仍只是部分为人所知。
