Gu Yiwen, Yan Huixiang, Bai Tianwen, Rao Daoyuan, Yang Yiwen, Hu Hongyu, Li Lei, Guo Longhua, Zeng Yanbo
College of Chemistry and Materials Sciences, Zhejiang Normal University, Jinhua, 321004, People's Republic of China.
Jiaxing Key Laboratory of Molecular Recognition and Sensing, College of Biological, Chemical Sciences and Engineering, Jiaxing University, Jiaxing, 314001, People's Republic of China.
Mikrochim Acta. 2025 Jan 10;192(2):64. doi: 10.1007/s00604-024-06907-y.
A novel Ru-FeO nanozyme with enhanced peroxidase-like (POD-like) activity was synthesized through a hydrothermal method. Ru-FeO nanozyme was effectively utilized for the detection of thiophanate-methyl (TM) using a colorimetric technique. The POD-like activity of Ru-FeO was found to be superior compared to FeO, Rh-FeO, and Pd-FeO. Ru-FeO provided excellent POD-like activity with Michaelis constants of 0.00645 mM and 0.66714 mM for substrates of 3,3',5,5'-tetramethylbenzidine and HO, respectively. The density functional theory calculation showed that Ru-FeO had better HO decomposition reactivity compared to FeO. Compared with FeO, the adsorbed HO molecules underwent decomposition more readily on the Ru-FeO catalytic surface facilitating the occurrence of the HO catalytic reaction, further suggesting the excellent POD-like activity of Ru-FeO. The coordination and electrostatic interactions of Ru-FeO and TM promoted their binding, contributing to TM covering Ru-FeO catalytic site and inhibiting the POD-like activity of Ru-FeO. As a result, the sensitive and selective detection of TM using Ru-FeO by a colorimetric method was achieved. The Ru-FeO nanozyme displayed a good linear correlation, with a linear detection range and a detection limit of 0.1-100 and 0.03 μg/mL, respectively. The Ru-FeO nanozyme was used for the determination of TM in soil and water samples with success.
通过水热法合成了一种具有增强类过氧化物酶（POD 样）活性的新型 Ru-FeO 纳米酶。利用比色技术，Ru-FeO 纳米酶被有效地用于检测甲基托布津（TM）。发现 Ru-FeO 的 POD 样活性优于 FeO、Rh-FeO 和 Pd-FeO。对于 3,3',5,5'-四甲基联苯胺和 H₂O₂底物，Ru-FeO 分别具有 0.00645 mM 和 0.66714 mM 的米氏常数，表现出优异的 POD 样活性。密度泛函理论计算表明，Ru-FeO 比 FeO 具有更好的 H₂O₂分解反应活性。与 FeO 相比，吸附在 Ru-FeO 催化表面的 H₂O₂分子更容易发生分解，促进了 H₂O₂催化反应的发生，进一步表明 Ru-FeO 具有优异的 POD 样活性。Ru-FeO 与 TM 的配位和静电相互作用促进了它们的结合，导致 TM 覆盖 Ru-FeO 催化位点并抑制 Ru-FeO 的 POD 样活性。结果，实现了用 Ru-FeO 通过比色法对 TM 的灵敏和选择性检测。Ru-FeO 纳米酶呈现出良好的线性相关性，线性检测范围和检测限分别为 0.1 - 100 μg/mL 和 0.03 μg/mL。Ru-FeO 纳米酶成功用于土壤和水样中 TM 的测定。
