Callihan D R, Nolte F S
Diagn Microbiol Infect Dis. 1985 Mar;3(2):131-7. doi: 10.1016/0732-8893(85)90022-7.
High-level clindamycin resistance in Bacteroides species was investigated by measuring zone sizes surrounding 2 micrograms clindamycin and 60 micrograms erythromycin discs, using a nonstandardized disc diffusion method, and by determining minimal inhibitory concentrations (MIC). The absence of a zone of inhibition surrounding either disc was predictive for all isolates having high-level resistance to both antibiotics (MIC greater than 256 micrograms/ml), characteristic of macrolide-lincosamide-streptogramin (MLS) cross-resistance. Although zone size could not be used as an absolute predictor of MIC, a clindamycin zone diameter of less than 17 mm was suggestive of strains with a moderate level of clindamycin resistance (MIC greater than or equal to 8 micrograms/ml), regardless of erythromycin zone size. Disc diffusion testing using a combination of clindamycin and erythromycin discs can be a useful screening method for detection of clindamycin-resistant Bacteroides species, occurring either alone or as part of the MLS resistance phenotype.
通过使用非标准化纸片扩散法测量围绕2微克克林霉素和60微克红霉素纸片的抑菌圈大小，并通过测定最低抑菌浓度（MIC），对拟杆菌属中的高水平克林霉素耐药性进行了研究。任一纸片周围缺乏抑菌圈可预测所有分离株对这两种抗生素均具有高水平耐药性（MIC大于256微克/毫升），这是大环内酯 - 林可酰胺 - 链阳菌素（MLS）交叉耐药的特征。虽然抑菌圈大小不能用作MIC的绝对预测指标，但无论红霉素抑菌圈大小如何，克林霉素抑菌圈直径小于17毫米提示菌株具有中等水平的克林霉素耐药性（MIC大于或等于8微克/毫升）。使用克林霉素和红霉素纸片组合的纸片扩散试验可作为检测单独出现或作为MLS耐药表型一部分的耐克林霉素拟杆菌属的有用筛查方法。
