Zhang Linsen, Yuan Xiu, Peng Qingmei
Department of Clinical laboratory, Yantaishan Hospital, No. 91 Jiefang Road, Yantai, Shandong 264000, China.
Department of obstetrical, The Affiliated Jiangning Hospital of Nanjing Medical University, No. 168, Gushan Road, Nanjing, Jiangsu 211100, China.
Toxicol Res (Camb). 2025 Jan 8;14(1):tfae235. doi: 10.1093/toxres/tfae235. eCollection 2025 Jan.
Ovarian cancer (OC) is a significant cause of cancer-related mortality among women. This study explores the efficacy of L. () extract, known for its phytoestrogenic properties, in treating OC through hormonal and metabolic modulation. Using a Wistar rat model, OC was induced with 7,12-dimethylbenz(a)anthracene (DMBA), and the effects of , both alone and in combination with paclitaxel (PTX), were evaluated. The study involved five groups of ten rats each: normal, OC, and those receiving 100 mg/kg of with or without PTX. Key hormonal levels, oxidative stress markers, and inflammatory cytokines were measured. Additionally, ovarian tissues were analyzed for malondialdehyde and ferric reducing ability of plasma, while gene and protein expressions related to apoptosis were assessed. Results showed that , particularly when combined with PTX, reduced the luteinizing hormone/follicle-stimulating hormone ratio, increased antioxidant enzyme activity, and upregulated apoptosis-related pathways, leading to higher p53 expression and fewer Ki-67 positive cells. These findings suggest 's potential as a complementary therapy for women with OC, particularly those with ovulation disorders.
卵巢癌（OC）是女性癌症相关死亡的一个重要原因。本研究探讨了以其植物雌激素特性而闻名的L.（）提取物通过激素和代谢调节治疗OC的疗效。使用Wistar大鼠模型，用7,12 - 二甲基苯并(a)蒽（DMBA）诱导OC，并评估其单独使用以及与紫杉醇（PTX）联合使用的效果。该研究涉及五组，每组十只大鼠：正常组、OC组以及接受100mg/kg的L.（）提取物且分别联合或不联合PTX的组。测量了关键激素水平、氧化应激标志物和炎性细胞因子。此外，分析了卵巢组织中的丙二醛和血浆铁还原能力，同时评估了与细胞凋亡相关的基因和蛋白质表达。结果表明，L.（）提取物，特别是与PTX联合使用时，降低了促黄体生成素/促卵泡激素比值，增加了抗氧化酶活性，并上调了细胞凋亡相关途径，导致p53表达升高且Ki-67阳性细胞减少。这些发现表明L.（）提取物作为OC女性，特别是排卵障碍女性的辅助治疗具有潜力。
