Villalva Marisol, Silvan Jose Manuel, Alarcón-Cavero Teresa, Villanueva-Bermejo David, Jaime Laura, Santoyo Susana, Martinez-Rodriguez Adolfo J
Microbiology and Food Biocatalysis Group (MICROBIO), Department of Biotechnology and Food Microbiology, Institute of Food Science Research (CIAL, CSIC-UAM), C/Nicolás Cabrera, 9. Cantoblanco Campus, Autonomous University of Madrid, 28049 Madrid, Spain.
Microbiology Department, Hospital Universitario de La Princesa, 28006 Madrid, Spain.
Antioxidants (Basel). 2022 Sep 20;11(10):1849. doi: 10.3390/antiox11101849.
The main objective of this work is to evaluate the potential utility of an extract (yarrow extract, YE) in the control of infection. The supercritical anti-solvent fractionation (SAF) process of YE allowed the obtaining of two different fractions: yarrow's precipitated fraction (YPF), enriched in most polar phenolic compounds (luteolin-7--glucoside, luteolin, and 3,5-dicaffeoylquinic acid), and yarrow's separator fraction (YSF), enriched in monoterpenes and sesquiterpenes, mainly containing camphor, artemisia ketone, and borneol. YE was effective in reducing reactive oxygen species (ROS) production in human gastric AGS cells by 16% to 29%, depending on the strain. YPF had the highest inhibitory activity (38-40%) for ROS production. YE modulated the inflammatory response in AGS gastric cells, decreasing IL-8 production by 53% to 64%. This IL-8 inhibition also showed a strain-dependent character. YPF and YSF exhibited similar behavior, reducing IL-8 production, suggesting that both phenolic compounds and essential oils could contribute to IL-8 inhibition. YSF showed the highest antibacterial activity against (6.3-7.1 log CFU reduction, depending on the strain) and lower MIC (0.08 mg/mL). Results obtained have shown that YE and SAF fractions (YPF and YSF) were effective as antioxidant, anti-inflammatory, and antibacterial agents regardless of the strain characteristics.
这项工作的主要目标是评估一种提取物（欧蓍草提取物，YE）在控制感染方面的潜在效用。YE的超临界抗溶剂分馏（SAF）过程得到了两种不同的馏分：欧蓍草沉淀馏分（YPF），富含大多数极性酚类化合物（木犀草素-7-葡萄糖苷、木犀草素和3,5-二咖啡酰奎宁酸）；以及欧蓍草分离馏分（YSF），富含单萜和倍半萜，主要含有樟脑、艾蒿酮和冰片。YE能有效降低人胃AGS细胞中活性氧（ROS）的产生，降低幅度为16%至29%，具体取决于菌株。YPF对ROS产生的抑制活性最高（38-40%）。YE调节AGS胃细胞中的炎症反应，使IL-8的产生减少53%至64%。这种对IL-8的抑制也表现出菌株依赖性。YPF和YSF表现出相似的行为，都能减少IL-8的产生，这表明酚类化合物和精油都可能有助于抑制IL-8。YSF对[具体细菌]表现出最高的抗菌活性（根据菌株不同，菌落形成单位减少6.3-7.1 log），且最低抑菌浓度（MIC）较低（0.08 mg/mL）。所获得的结果表明，无论菌株特征如何，YE和SAF馏分（YPF和YSF）作为抗氧化剂、抗炎剂和抗菌剂都是有效的。
