Sharma Priyamvada, Rao Vijayashree, Shukla Lekhansh, Murthy Pratima
Department of Clinical Psychopharmacology and Neurotoxicology, National Institute of Mental Health and Neurosciences, Bangalore, IND.
Centre for Addiction Medicine, Department of Psychiatry, National Institute of Mental Health and Neurosciences, Bangalore, IND.
Cureus. 2024 Dec 9;16(12):e75424. doi: 10.7759/cureus.75424. eCollection 2024 Dec.
Background The synthetic opioid tramadol is widely used as a pain reliever. Unlike other opioids, it is used freely worldwide, unaffected by international controls resulting in abuse and accidental intoxication. Analytical methods are necessary to prove tramadol abuse because 30% of the drug is excreted unchanged. Methodology This study describes a sensitive, precise, and accurate gas chromatograph-mass spectrometry (GC-MS) method for tramadol quantification in biological samples using solid-phase extraction (SPE) for sample preparation. Results A total of 747 samples were analyzed for suspected tramadol abuse; 15% of samples were above cut-off with a mean of 341.0 ± 215 ng/mL. No interference from other substances was detected. Calibration was linear over the concentration range of 50-1,000 ng/mL with a correlation coefficient of >0.998. Recovery was 92.5% and precision was ≤5% (range = 2.68-5.58%). Conclusions The effectiveness of the SPE in assessing tramadol abuse was assessed with GC-MS. With good recovery, quick analysis times, simplicity, little matrix effect, and effectiveness, this method is regarded as novel and is capable of identifying abuse.
合成阿片类药物曲马多被广泛用作止痛剂。与其他阿片类药物不同，它在全球范围内可自由使用，不受国际管制影响，这导致了滥用和意外中毒。由于30%的药物以原形排泄，因此需要分析方法来证明曲马多滥用情况。
本研究描述了一种灵敏、精确且准确的气相色谱-质谱联用（GC-MS）方法，用于生物样品中曲马多的定量分析，采用固相萃取（SPE）进行样品制备。
共分析了747份疑似曲马多滥用的样品；15%的样品超过临界值，平均浓度为341.0±215 ng/mL。未检测到其他物质的干扰。在50-1000 ng/mL的浓度范围内校准呈线性，相关系数>0.998。回收率为92.5%，精密度≤5%（范围=2.68-5.58%）。
采用GC-MS评估了SPE在评估曲马多滥用方面的有效性。该方法回收率良好、分析时间短、操作简单、基质效应小且有效，被认为是一种新颖的方法，能够识别滥用情况。
