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濒危木腐真菌Occarium somion的核基因组和线粒体基因组组装

Nuclear and Mitochondrial Genome Assemblies for the Endangered Wood-Decaying Fungus Somion occarium.

作者信息

Hill Rowena, McGowan Jamie, Brabcová Vendula, McTaggart Seanna, Irish Naomi, Barker Tom, Knitlhoffer Vanda, Lucchini Sacha, Baker Kendall, Catchpole Leah, Watkins Chris, Gharbi Karim, Kaithakottil Gemy, Tracey Alan, Wood Jonathan M D, Tomšovský Michal, Baldrian Petr, Swarbreck David, Hall Neil

机构信息

Earlham Institute, Norwich, UK.

Laboratory of Environmental Microbiology, Institute of Microbiology of the Czech Academy of Sciences, Prague, Czech Republic.

出版信息

Genome Biol Evol. 2025 Jan 6;17(1). doi: 10.1093/gbe/evaf003.

Abstract

Somion occarium is a wood-decaying bracket fungus belonging to an order known to be rich in useful chemical compounds. Despite its widespread distribution, S. occarium has been assessed as endangered on at least 1 national Red List, presumably due to loss of old-growth forest habitat. Here, we present a near-complete, annotated nuclear genome assembly for S. occarium consisting of 31 Mbp arranged in 11 pseudochromosomes-9 of which are telomere-to-telomere-as well as a complete mitochondrial genome assembly of 112.9 Kbp. We additionally performed phylogenomic analysis and annotated carbohydrate-active enzymes (CAZymes) to compare gene and CAZyme content across closely related species. This genome was sequenced as the representative for Kingdom Fungi in the European Reference Genome Atlas Pilot Project.

摘要

枕状拟层孔菌是一种腐朽木材的多孔菌,属于一个已知富含有用化合物的目。尽管其分布广泛,但枕状拟层孔菌在至少一份国家红色名录中被评估为濒危物种,可能是由于原始森林栖息地的丧失。在此,我们展示了一个近乎完整的、有注释的枕状拟层孔菌核基因组组装,其由31兆碱基对组成,排列在11条假染色体中,其中9条是端粒到端粒的,以及一个112.9千碱基对的完整线粒体基因组组装。我们还进行了系统基因组分析并注释了碳水化合物活性酶(CAZymes),以比较密切相关物种间的基因和CAZyme含量。该基因组作为欧洲参考基因组图谱试点项目中真菌界的代表进行了测序。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/563e/11783313/ebb9241d9eb6/evaf003f1.jpg

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